金属离子对脂肪酶 Candida sp. 99-125 水解麻籽油的影响。

Effect of metal ions on the enzymatic hydrolysis of hemp seed oil by lipase Candida sp. 99-125.

机构信息

School of Life Sciences, Zhengzhou University, Zhengzhou, Henan, China.

出版信息

Int J Biol Macromol. 2018 Jul 15;114:922-928. doi: 10.1016/j.ijbiomac.2018.03.168. Epub 2018 Mar 28.

DOI:10.1016/j.ijbiomac.2018.03.168

Abstract

In order to study the effect of metal ions on the enzymatic hydrolysis of hemp seed oil by Candida sp. 99-125, the spectroscopy, stability and hydrolytic activity of the biocatalyst were investigated in presence of Ca, Mg, Fe, Fe, Cu, Sn, Pb, Zn and Ba metal ions, respectively. The UV spectroscopy showed that all the metal ions enhanced the absorbance but the decrease of fluorescence intensity was observed. All the metal ions could improve the lipase thermal stability except Cu and Ba. Hydrolysis of hemp seed oil proved that Ca, Fe, Pb and Ba could significantly improve the hydrolytic rate, and metal ions could influence lipase selectivity. The study revealed that metal ions could improve lipase stability, hydrolysis activity in the hydrolytic process of hemp seed oil by Candida sp. 99-125.

摘要

为了研究金属离子对 Candida sp. 99-125 酶解麻籽油的影响，分别在 Ca、Mg、Fe、Fe、Cu、Sn、Pb、Zn 和 Ba 金属离子存在下，研究了生物催化剂的光谱、稳定性和水解活性。紫外光谱表明，所有金属离子均增强了吸光度，但观察到荧光强度下降。除 Cu 和 Ba 外，所有金属离子均可提高脂肪酶的热稳定性。麻籽油的水解证明，Ca、Fe、Pb 和 Ba 可显著提高水解速率，金属离子可影响脂肪酶的选择性。研究表明，金属离子可以提高脂肪酶的稳定性，从而提高 Candida sp. 99-125 在麻籽油水解过程中的水解活性。