M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997, Miklukho-Maklaya 16/10, Moscow, Russia; Scientific Research Centre "Crystallography and Photonics" of the Russian Academy of Sciences, 119333, Leninsky pr. 59, Moscow, Russia.
M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997, Miklukho-Maklaya 16/10, Moscow, Russia.
Colloids Surf B Biointerfaces. 2018 Jun 1;166:303-322. doi: 10.1016/j.colsurfb.2018.03.036. Epub 2018 Mar 26.
Dispersions of polymer particle (DPPs) are increasingly being exploited both as biomolecule carriers, and as markers in various DPP biomedical applications related to cell and molecular biology, enzymology, immunology, diagnostics, in vitro and in vivo visualization, bioseparation, etc. Their potential to reduce reaction scales, lower costs, improve the rate, sensitivity, selectivity, stability and reproducibility of assays governs the diversity of their bioapplications. This review focuses on the design of DPPs with innovative special properties in the course of free radical heterophase polymerization that provides careful control of both macromolecular and colloidal properties. We demonstrate approaches that, according to the polymerization technique, regulate the particle size, shape, particle size distribution, morphology, surface chemistry and functionality, as well as the formation of organic-inorganic hybrid DPPs. The production of bioreagents based on DPPs and their use in bioassay are also reviewed.
聚合物粒子分散体（DPPs）越来越多地被用作生物分子载体，以及与细胞和分子生物学、酶学、免疫学、诊断学、体外和体内可视化、生物分离等相关的各种 DPP 生物医学应用中的标记物。它们具有降低反应规模、降低成本、提高分析物的速率、灵敏度、选择性、稳定性和重现性的潜力，这决定了它们生物应用的多样性。本综述重点介绍了在自由基杂相聚合过程中设计具有创新特殊性质的 DPPs，该聚合过程可对大分子和胶体性质进行精细控制。我们展示了根据聚合技术调节粒径、形状、粒径分布、形态、表面化学和功能以及有机-无机杂化 DPPs 形成的方法。还综述了基于 DPPs 的生物试剂的生产及其在生物测定中的应用。
