Micklem L R, McCann M C, James K
Department of Surgery, University of Edinburgh Medical School, U.K.
J Immunol Methods. 1987 Nov 23;104(1-2):81-6. doi: 10.1016/0022-1759(87)90490-x.
Allogeneic rat mixed-thymocyte 48 h culture-conditioned medium (MTM) was used successfully in place of feeder cells for hybridoma production with the NS-1 and NS-0 plasmacytoma lines. It permitted lower concentrations of fused cells to be seeded, and supported the transition from 96 to 24 well plates. MTM improved the performance of poor sera during cloning. It also assisted the survival of cells that were sensitive to thawing from liquid nitrogen storage, and cells that had inadvertently been allowed to overgrow. Two rats could produce the equivalent of 1500-5000 ml feeder cell suspension according to the dilution used; 150-500 mice would be required to produce such a quantity of cells. Thus use of MTM entailed a considerable saving in mice and provided a secure supply of 'reagent', since a batch could be prepared, checked for sterility, frozen and stored indefinitely.
异基因大鼠混合胸腺细胞48小时培养条件培养基（MTM）成功地替代了饲养细胞，用于与NS-1和NS-0骨髓瘤细胞系进行杂交瘤生产。它允许接种较低浓度的融合细胞，并支持从96孔板过渡到24孔板。MTM在克隆过程中改善了劣质血清的性能。它还帮助了对从液氮储存中解冻敏感的细胞以及无意中过度生长的细胞存活。根据使用的稀释度，两只大鼠可产生相当于1500 - 5000毫升饲养细胞悬液；产生如此数量的细胞则需要150 - 500只小鼠。因此，使用MTM可大量节省小鼠，并提供“试剂”的安全供应，因为一批MTM可以制备、检查无菌性、冷冻并无限期储存。
