Terao Yasuo, Fukuda Hideki, Sugiyama Yusuke, Inomata-Terada Satomi, Tokushige Shin-Ichi, Hamada Masashi, Ugawa Yoshikazu
Department of Cell Physiology, Kyorin University;
Segawa Memorial Neurological Clinic for Children.
J Vis Exp. 2018 Mar 13(133):56934. doi: 10.3791/56934.
Electro-oculogram (EOG) has been widely used for clinical eye movement recording, especially horizontal saccades, although the video-oculography (VOG) has largely taken the place of it nowadays due to its higher spatial accuracy. However, there are situations in which EOG has clear advantages over VOG, e.g., subjects with narrow eye clefts or having cataract lenses, and patients with movement disorders. The present article shows that if properly implemented, EOG can achieve an accuracy almost as good as VOG with substantial stability for recording, while circumventing problems associated with VOG recording. The present paper describes a practical method for recording horizontal saccades using oculomotor paradigms with high accuracy and stability by EOG in neurological patients. The necessary measures are to use an Ag-AgCl electrode with a wide plastic fringe capable of reducing noise, and to wait for sufficient light adaptation to occur. This waiting period also helps to lower the impedance between the electrodes and the skin, thereby ensuring stable signal recorded as time goes by. Furthermore, re-calibration is performed as needed during the task performance. Using this method, the experimenter can avoid drifts of signals, as well as contamination of artifacts or noise from the electromyogram and electroencephalogram, and can collect sufficient data for clinical evaluation of saccades. Thus when implemented, EOG can still be a method of high practicability that can be widely applied to neurological patients, but may be effective also for studies in normal subjects.
眼电图（EOG）已被广泛用于临床眼动记录，尤其是水平扫视，尽管如今视频眼动图（VOG）因其更高的空间精度在很大程度上已取代了它。然而，在某些情况下，EOG比VOG具有明显优势，例如睑裂狭窄或患有白内障晶状体的受试者，以及患有运动障碍的患者。本文表明，如果实施得当，EOG可以实现几乎与VOG一样好的精度，并且在记录时具有相当的稳定性，同时避免与VOG记录相关的问题。本文描述了一种在神经科患者中使用眼动范式通过EOG高精度、稳定地记录水平扫视的实用方法。必要的措施是使用带有宽塑料边缘的Ag - AgCl电极以减少噪声，并等待足够的光适应发生。这个等待期也有助于降低电极与皮肤之间的阻抗，从而确保随着时间推移记录的信号稳定。此外，在任务执行过程中根据需要进行重新校准。使用这种方法，实验者可以避免信号漂移，以及来自肌电图和脑电图的伪迹或噪声污染，并可以收集足够的数据用于扫视的临床评估。因此，当实施时，EOG仍然是一种具有高度实用性的方法，可以广泛应用于神经科患者，但对正常受试者的研究可能也有效。
