Roxburgh A E, Cooper I A
Haematology Research Unit, Peter MacCallum Cancer Institute, Melbourne, Victoria, Australia.
Leuk Res. 1987;11(10):891-901. doi: 10.1016/0145-2126(87)90135-4.
Patterns of leucocyte-common antigen (L-CA) and large sialoglycoprotein (LSGP) expression on leukemic peripheral blood lymphocytes of 13 patients with chronic lymphocytic leukemia (CLL), 17 with non-Hodgkin's lymphoma (NHL) in leukemic phase and one with hairy cell leukemia (HCL) have been examined by means of surface labelling and electrophoresis in 5% polyacrylamide gels. The 13 CLL, 10 of the 11 diffuse NHL and the six nodular poorly differentiated lymphocytic lymphoma (PDLL) patients fell into three groups according to expression of 210, 198 and 185k forms of L-CA. Group 1 (210 less than 198 less than 185k L-CA) included eight CLL and one diffuse NHL; Group 2 (210 greater than or equal to 198 and 185k L-CA) included four CLL, three diffuse NHL and four nodular PDLL; Group 3 (mainly 210k L-CA) included one CLL, six diffuse NHL and two nodular PDLL. A patient with diffuse large cell lymphoma and the HCL patient both had patterns of multiple, diffuse, very high Mr labelled glycoproteins. LSGP on these cells varied from nil to very high and levels were not related to L-CA patterns. Lymph node cells from five patients were also studied and were found to express larger numbers of L-CA forms and less LSGP than corresponding peripheral blood lymphocytes. Possible relationships of L-CA forms and LSGP to lymphocyte function and disease patterns are discussed.
通过表面标记和在5%聚丙烯酰胺凝胶中进行电泳，检测了13例慢性淋巴细胞白血病（CLL）患者、17例处于白血病期的非霍奇金淋巴瘤（NHL）患者和1例毛细胞白血病（HCL）患者白血病外周血淋巴细胞上白细胞共同抗原（L-CA）和大唾液酸糖蛋白（LSGP）的表达模式。根据L-CA的210k、198k和185k形式的表达情况，13例CLL患者、11例弥漫性NHL患者中的10例以及6例结节性低分化淋巴细胞淋巴瘤（PDLL）患者分为三组。第1组（210k L-CA < 198k L-CA < 185k L-CA）包括8例CLL患者和1例弥漫性NHL患者；第2组（210k L-CA ≥ 198k L-CA且210k L-CA ≥ 185k L-CA）包括4例CLL患者、3例弥漫性NHL患者和4例结节性PDLL患者；第3组（主要为210k L-CA）包括1例CLL患者、6例弥漫性NHL患者和2例结节性PDLL患者。1例弥漫性大细胞淋巴瘤患者和HCL患者均具有多种、弥漫性、高分子量标记糖蛋白的模式。这些细胞上的LSGP从无到非常高，其水平与L-CA模式无关。还研究了5例患者的淋巴结细胞，发现其表达的L-CA形式数量更多，而LSGP比相应的外周血淋巴细胞少。讨论了L-CA形式和LSGP与淋巴细胞功能及疾病模式的可能关系。
