伊拉克人群中与2型糖尿病相关基因的rs1801282（G/C）多态性如何？
Is the rs1801282 (G/C) Polymorphism of Gene Associated with T2DM in Iraqi People?
作者信息
Al-Naemi Amjad Hazim, Ahmad Akram Jarjees
机构信息
Department of Biochemistry, Mosul Medical College, University of Mosul, Mosul, Iraq.
出版信息
Open Access Maced J Med Sci. 2018 Mar 14;6(3):447-455. doi: 10.3889/oamjms.2018.156. eCollection 2018 Mar 15.
BACKGROUND
Pro12Ala (rs1801282) is a common polymorphism of the human PPAR-γ gene. Studies have demonstrated conflicting results about its association with T2DM worldwide. There are no reports about such possible association among Iraqi people.
OBJECTIVES
This study aims at finding out whether having the mutant allele (Ala12) might be associated with T2DM among Iraqi people.
METHODS
One hundred and ninety-two Arabic Iraqi adult subjects (97 with T2DM and 95 controls) were genotyped using PCR- RFLP. Clinical, anthropometrical and biochemical variables were compared regarding the Pro12Ala genotypes.
RESULTS
About 5.67% of people with diabetes were carriers of the (Ala12) allele versus 9.47% of controls. Allelic and genotypic frequencies were not statistically different among diabetics and controls [(χ= 1.99, p= 0.16) and (χ= 2.17, p= 0.14)]. Age, BMI and smoking- but not Pro12Ala - were independent risk factors for T2DM in our subjects. Pro12Ala was not associated with T2DM (Odd's ratio 0.55, 95% CI 0.23- 1.32, p= 0.14).
CONCLUSIONS
Our study revealed a relatively high frequency of the Ala12 allele among Arabic Iraqis. These frequencies did not significantly differ between diabetics and controls indicating the absence of association of Pro12Ala with T2DM among Iraqis.
背景
Pro12Ala（rs1801282）是人类PPAR-γ基因的一种常见多态性。世界各地关于其与2型糖尿病关联的研究结果相互矛盾。伊拉克人群中尚无此类可能关联的报道。
目的
本研究旨在探究携带突变等位基因（Ala12）是否与伊拉克人群的2型糖尿病相关。
方法
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对192名成年伊拉克阿拉伯受试者（97例2型糖尿病患者和95例对照）进行基因分型。比较了Pro12Ala基因型的临床、人体测量和生化变量。
结果
糖尿病患者中约5.67%为（Ala12）等位基因携带者，而对照组为9.47%。糖尿病患者和对照组之间的等位基因和基因型频率无统计学差异[（χ=1.99，p=0.16）和（χ=2.17，p=0.14）]。在我们的研究对象中，年龄、体重指数和吸烟（而非Pro12Ala）是2型糖尿病的独立危险因素。Pro12Ala与2型糖尿病无关（优势比0.55，95%可信区间0.23 - 1.32，p=0.14）。
结论
我们的研究显示伊拉克阿拉伯人中Ala12等位基因频率相对较高。糖尿病患者和对照组之间这些频率无显著差异，表明伊拉克人中Pro12Ala与2型糖尿病无关联。
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