Department of Ophthalmology, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, China.
Invest Ophthalmol Vis Sci. 2018 Apr 1;59(5):1802-1809. doi: 10.1167/iovs.17-23660.
To explore the quantitative distributions of different lymphocyte subsets in the lacrimal sac mucosa and identify changes of Th1- and Th2-associated cytokines in the tears of patients with chronic dacryocystitis.
Lacrimal sac mucosal specimens from patients with chronic dacryocystitis were analyzed. Hematoxylin-eosin staining and Masson staining were performed for pathologic analysis, and immunohistochemical staining was performed for the detection of CD3+, CD4+, CD8+, CD20+, Th1, and Th2 lymphocytes. Quantitative real-time PCR was performed to analyze IFN-γ and IL-4 mRNA expression. In addition, tear samples from patients with chronic dacryocystitis and healthy volunteers were collected and analyzed with an antibody array system for Th1- and Th2-associated cytokines and chemokines.
Different distribution patterns of lymphocyte subsets were observed in the lacrimal sac walls. Both CD20+ B lymphocytes and CD3+ T lymphocytes accumulated in organized lymphoid follicles, and CD3+ T cells were also distributed in a diffuse manner. Among the two subsets of T cells, CD4+ T cells were more abundant than CD8+ T cells. Both the immunohistochemical staining and real-time PCR results revealed significantly higher expression levels of IFN-γ than those of IL-4. The levels of Th1- and Th2-related cytokines and chemokines measured were significantly higher in the tears of patients than in those of controls.
The different distribution patterns of lymphocyte subsets provide insight into a potential immunologic mechanism for dacryocystitis. The cytokines secreted by Th1 or Th2 cells may play a major role in the pathogenesis of dacryocystitis and could be explored as therapeutic targets.
探讨不同淋巴细胞亚群在泪囊黏膜中的定量分布，并鉴定慢性泪囊炎患者泪液中 Th1 和 Th2 相关细胞因子的变化。
分析慢性泪囊炎患者的泪囊黏膜标本。进行苏木精-伊红染色和 Masson 染色进行病理分析，并进行免疫组织化学染色以检测 CD3+、CD4+、CD8+、CD20+、Th1 和 Th2 淋巴细胞。采用实时定量 PCR 分析 IFN-γ 和 IL-4 mRNA 表达。此外，收集慢性泪囊炎患者和健康志愿者的泪液样本，并使用抗体阵列系统分析 Th1 和 Th2 相关细胞因子和趋化因子。
观察到泪囊壁中淋巴细胞亚群的不同分布模式。CD20+B 淋巴细胞和 CD3+T 淋巴细胞均在有组织的淋巴滤泡中积聚，并且 CD3+T 细胞也呈弥漫性分布。在这两个 T 细胞亚群中，CD4+T 细胞比 CD8+T 细胞更丰富。免疫组织化学染色和实时 PCR 结果均显示 IFN-γ 的表达水平明显高于 IL-4。患者泪液中 Th1 和 Th2 相关细胞因子和趋化因子的水平明显高于对照组。
淋巴细胞亚群的不同分布模式为泪囊炎的潜在免疫学机制提供了深入了解。Th1 或 Th2 细胞分泌的细胞因子可能在泪囊炎的发病机制中起主要作用，并可作为治疗靶点进行探索。
