二甲基亚砜诱导分化过程中弗氏红白血病细胞翻译控制机制的改变

Alterations in translational control mechanisms in Friend erythroleukemic cells during DMSO induced differentiation.

作者信息

Koch G, Weber C, Bilello J A

出版信息

Haematol Blood Transfus. 1979;23:295-301. doi: 10.1007/978-3-642-67057-2_37.

Abstract

Induction of erythroid differentiation in Friend erythroleukemia cells causes a reduction in the rate of protein synthesis and a prolonged growth cycle of the cells. A study of the relative translational efficiencies (RTE) of viral mRNAs and selected host cell mRNAs revealed unexpected low RTEs of the mRNA coding for the gp70 protein and RTE of the mRNA coding for the p30 protein. The RTEs of viral and of some host mRNAs show significant changes during differentiation. The rate of synthesis of corresponding proteins are altered non-coordinately, indicating transcriptional and/or posttranscriptional regulation of mRNA, as well as amplified regulation at the level of translation of mRNA.

摘要

弗氏红白血病细胞中红细胞分化的诱导会导致蛋白质合成速率降低以及细胞生长周期延长。对病毒mRNA和选定宿主细胞mRNA的相对翻译效率（RTE）进行的一项研究显示，编码gp70蛋白的mRNA的RTE出人意料地低，而编码p30蛋白的mRNA的RTE也很低。病毒mRNA和一些宿主mRNA的RTE在分化过程中显示出显著变化。相应蛋白质的合成速率发生了非协调性改变，这表明mRNA存在转录和/或转录后调控，以及在mRNA翻译水平上的放大调控。