Gauquelin G, Kazek C, Allevard A M, Garcin R, Bonnod J, Gutkowska J, Cantin M, Gharib C
Lab. Physiol. Fac. Med. Grange-Blanche, Lyon, France.
Biochem Biophys Res Commun. 1987 Oct 29;148(2):582-8. doi: 10.1016/0006-291x(87)90916-8.
Antiorthostatic hypokinetic suspension (AOH) in rat is currently used as an animal model for simulating weightlessness. This maneuver is responsible for a diuresis, a natriuresis and an increase in central venous pressure (CVP). Knowing the role of CVP in atrial natriuretic factor (ANF) secretion, the aim of the study was to examine the early plasma ANF changes during AOH (angle 30-35 degrees) using Morey's model (tail suspension). The rats were divided into 4 groups: 24 population cage (PC), 24 isolated in separate cages (I), 24 were attached by the tail (Morey's model) and remained in the horizontal position (attached horizontal: AH). At the end of this period of 7 d, 12 AH were suspended for 1, 2, 6 and 24 h (AOH) and sacrificed with the controls for plasma ANF determination. Our results show that the level of ANF is significantly (p less than .05) higher in AOH rats after 2 h of suspension (16.6 +/- 2 pg/ml vs 10.9 +/- 1.5). A significant increase is also observed between AOH and AH after 2h of suspension (p less than 0.05). Six hours after suspension ANF presents a sharp decline in AOH and no difference is observed between AOH and AH and I. Morey's tail suspension model seems to be valid for the study of the early hormonal effects of simulated weightlessness for ANF.
大鼠抗立位低动力悬吊（AOH）目前被用作模拟失重的动物模型。这种操作会导致利尿、利钠以及中心静脉压（CVP）升高。鉴于CVP在心房利钠因子（ANF）分泌中的作用，本研究的目的是使用莫雷模型（尾部悬吊）来检测AOH（角度30 - 35度）期间血浆ANF的早期变化。将大鼠分为4组：24只饲养在群体笼中（PC），24只单独饲养在分隔笼中（I），24只通过尾巴悬吊（莫雷模型）并保持水平位置（附着水平：AH）。在7天的这段时间结束时，12只AH大鼠被悬吊1、2、6和24小时（AOH），并与对照组一起处死以测定血浆ANF。我们的结果表明，悬吊2小时后，AOH大鼠的ANF水平显著升高（p < 0.05）（16.6 ± 2 pg/ml对10.9 ± 1.5）。悬吊2小时后，AOH组与AH组之间也观察到显著升高（p < 0.05）。悬吊6小时后，AOH组的ANF急剧下降，AOH组与AH组和I组之间未观察到差异。莫雷的尾部悬吊模型似乎对于研究模拟失重对ANF的早期激素效应是有效的。
