Allen D P, Low P S, Dola A, Maisel H
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907.
Biochem Biophys Res Commun. 1987 Nov 30;149(1):266-75. doi: 10.1016/0006-291x(87)91634-2.
Although immunological homologues of erythrocyte membrane proteins have been individually discovered in a wide variety of tissues and cultured cells, the major structural components of the membrane have not yet been demonstrated simultaneously in the same cell type. Thus, considerable uncertainty continues to exist concerning whether the red cell homologues form elements of a structure which is similar to or unique from the framework which supports the erythrocyte membrane. Because the red cell cytoskeletal proteins, spectrin, actin and band 4.1, have been previously found in the superficial cortex of the lens, we decided to determine whether the corresponding membrane anchoring components of band 3 and ankyrin also occur in this cell type. Using antiserum specific for band 3 and ankyrin, we report the existence of immunologically cross-reactive proteins of similar molecular weight. Because these anchoring proteins appear and disappear coordinately with the aforementioned cytoskeletal proteins during the intermediate stages of lens cell maturation, it is conceivable that an erythrocyte-like membrane structural organization may occur transiently in the eye lens.
尽管在多种组织和培养细胞中已分别发现红细胞膜蛋白的免疫学同源物，但膜的主要结构成分尚未在同一细胞类型中同时得到证实。因此，关于红细胞同源物是否构成与支持红细胞膜的框架相似或独特的结构元件，仍然存在相当大的不确定性。由于先前已在晶状体的表层皮质中发现红细胞细胞骨架蛋白血影蛋白、肌动蛋白和带4.1，我们决定确定带3和锚蛋白相应的膜锚定成分是否也存在于这种细胞类型中。使用针对带3和锚蛋白的特异性抗血清，我们报告了存在分子量相似的免疫交叉反应蛋白。由于这些锚定蛋白在晶状体细胞成熟的中间阶段与上述细胞骨架蛋白协同出现和消失，可以想象在眼晶状体中可能会短暂出现类似红细胞的膜结构组织。
