Mou J J, Qi M Y, Li C S, Zheng H Y, Chen M, Zhou J H, He J L, Xu W W, Xu S T, Xu X G
Institute of Integrative Medicine of Dalian Medical University, Dalian 116044, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2018 Apr 6;52(4):419-423. doi: 10.3760/cma.j.issn.0253-9624.2018.04.016.
To analyze the genetic characterization of glycoprotein M(gM.),glycoprotein L(gL) of varicella zoster virus. According to the program of "Ministry of Science and Technology of China" , Based on the 12 suspected VZV patients monitored in Beijing (1 case), Shanghai (5 cases), Jilin (2 cases), Qinghai (1 case), Guangdong (2 case) and Sichuan (case) in 2007-2015. A total of 12 Vesicle fluid and throat swab samples were collected. Positive samples were identified by Agarose gel electrophoresis and two glycoprotein genes were amplified by polymerase chain reaction (PCR). Nucleotide sequences were determined and analyzed by PCR amplification of VZV positive specimens V-OKA-BK of the domestic varicella attenuated live vaccine and the Varilrix-1 of the imported attenuated live vaccine. Nucleotide sequences of VZV positive specimens, vaccine strains (V-OKA-BK, varilrix-1) and GenBank foreign wild strains (41 strains), parent strains (P-oka), vaccine strains (V-oka, Varilrix, Varivax) were compared using BioEdit and MEGA 5.0. 12 specimens were VZV positive. Compared with the vaccine strains and the parent strains, the GM gene of 1 positive specimen had radical mutation at 86686 sites, which resulted in amino acid mutation, 5 positive specimens had base mutation at 87844 sites, and 30 strains of foreign wild strains had the same variation at 87 844 sites. 1 positive specimens of gL gene in 101245 sites had base mutation, and led to amino acid mutation, 6 positive specimens at 101624, 101625, 101626 sites had base of loss and the foreign wild strains in these 3 sites had the same variation. Compared with the vaccine strains, the nucleotide and amino acid homology of gM of 12 VZV positive specimens were 99.2%-100% and 98.2%-100%, respectively, and gL of those were 99.3%-100% and 98.6%-100%, respectively. Compared with 41 strains of foreign wild strains, homology of gM's nucleotides and amino acid were 99.3%-100% and 98.5%-100%, respectively; 99.1%-100% and 98.6%-100% for gL. The results of phylogenetic analysis showed that 7 VZV positive samples were on the same branch with 4 vaccine strains and p-oka strain. Based on gL, 12 VZV positive samples were on the same branch as the vaccine strains and p-oka strain. This study demonstrates that the genes of gM, gL are highly conserved and remain stable immunogen, which may be involved in the attenuation of VZV and need to be further researched.
分析水痘带状疱疹病毒糖蛋白M（gM）、糖蛋白L（gL）的基因特征。根据“中国科学技术部”项目，基于2007 - 2015年在北京（1例）、上海（5例）、吉林（2例）、青海（1例）、广东（2例）和四川（1例）监测的12例疑似水痘带状疱疹病毒（VZV）患者。共采集12份水疱液和咽拭子样本。通过琼脂糖凝胶电泳鉴定阳性样本，并通过聚合酶链反应（PCR）扩增两个糖蛋白基因。对VZV阳性标本、国产水痘减毒活疫苗的V - OKA - BK及进口减毒活疫苗的Varilrix - 1进行PCR扩增，测定并分析核苷酸序列。使用BioEdit和MEGA 5.0比较VZV阳性标本、疫苗株（V - OKA - BK、varilrix - 1）以及GenBank国外野生株（41株）、亲代株（P - oka）、疫苗株（V - oka、Varilrix、Varivax）的核苷酸序列。12份标本为VZV阳性。与疫苗株和亲代株相比，1份阳性标本的gM基因在86686位点发生根本突变，导致氨基酸突变，5份阳性标本在87844位点发生碱基突变，30株国外野生株在87844位点有相同变异。1份gL基因阳性标本在101245位点发生碱基突变，导致氨基酸突变，6份阳性标本在101624、101625、101626位点发生碱基缺失，国外野生株在这3个位点有相同变异。与疫苗株相比，12份VZV阳性标本的gM核苷酸和氨基酸同源性分别为99.2% - 100%和98.2% - 100%，gL分别为99.3% - 100%和98.6% - 100%。与41株国外野生株相比，gM核苷酸和氨基酸同源性分别为99.3% - 100%和98.5% - 100%；gL分别为99.1% - 100%和98.6% - 100%。系统发育分析结果显示，7份VZV阳性样本与4株疫苗株和p - oka株在同一分支上。基于gL，12份VZV阳性样本与疫苗株和p - oka株在同一分支上。本研究表明，gM、gL基因高度保守且免疫原性稳定，可能参与VZV的减毒过程，有待进一步研究。
需注意，原文中“Sichuan (case)”表述有误，推测应为“Sichuan (1 case)”，译文已按此修正。
