Sasaki Takahiro, Seki Junji, Itano Tomoaki, Sugihara-Seki Masako
Kansai University, Suita, Japan.
Osaka University, Toyonaka, Japan.
Biorheology. 2018;54(5-6):153-165. doi: 10.3233/BIR-18166.
In the microcirculation, red blood cells (RBCs) were observed to be confined to an axial stream surrounded by a marginal RBC depleted layer. This axial accumulation of RBCs is considered to arise from the RBC deformability.
To quantitatively evaluate the effect of RBC deformability on their axial accumulation at a flow condition comparable to that in arterioles by developing a new observation system for accurate measurements of radial RBC positions in the cross section of capillary tubes.
The cross-sectional distributions of normal and hardened RBCs as well as softened RBCs suspended in capillary tube flows were measured with high spatial resolution. A new observation system was developed in which enface views of the cross-section of the tube were obtained at small distances upstream of the outlet at various longitudinal positions in the tube.
The radial positions of individual RBCs were detected within 1 μm accuracy. It was found that normal and softened RBCs rapidly migrated away from the wall towards the tube axis, whereas glutaraldehyde-hardened RBCs were dispersed widely over the tube cross-section, depending on the concentration of glutaraldehyde solution.
The newly devised observation system revealed quantitatively the essential role of RBC deformability in their axial accumulation.
在微循环中，观察到红细胞（RBCs）局限于由边缘红细胞耗尽层包围的轴流中。红细胞的这种轴向聚集被认为是由红细胞的可变形性引起的。
通过开发一种新的观察系统，以精确测量毛细管横截面中红细胞的径向位置，在与小动脉中流动条件相当的情况下，定量评估红细胞可变形性对其轴向聚集的影响。
以高空间分辨率测量悬浮在毛细管流中的正常红细胞、硬化红细胞以及软化红细胞的横截面分布。开发了一种新的观察系统，在该系统中，在管内不同纵向位置的出口上游小距离处获得管横截面的正面视图。
单个红细胞的径向位置检测精度在1μm以内。发现正常和软化的红细胞迅速从管壁向管轴迁移，而戊二醛硬化的红细胞则根据戊二醛溶液的浓度在管横截面上广泛分散。
新设计的观察系统定量揭示了红细胞可变形性在其轴向聚集中的重要作用。
