Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of the Russian Academy of Sciences, premises 8, building 1, Village of Institute of Poliomyelitis, Settlement "Moskovskiy", 108819 Moscow, Russia.
Institute for Translational Medicine and Biotechnology, Sechenov First Moscow State Medical University, 8 Trubetskaya St., 119991 Moscow, Russia.
Viruses. 2018 Mar 31;10(4):164. doi: 10.3390/v10040164.
Wild ducks are known to be able to carry avian influenza viruses over long distances and infect domestic ducks, which in their turn infect domestic chickens. Therefore, prevention of virus transmission between ducks and chickens is important to control the spread of avian influenza. Here we used a low pathogenic wild aquatic bird virus A/duck/Moscow/4182/2010 (H5N3) for prevention of highly pathogenic avian influenza virus (HPAIV) transmission between ducks and chickens. We first confirmed that the ducks orally infected with H5N1 HPAIV A/chicken/Kurgan/3/2005 excreted the virus in feces. All chickens that were in contact with the infected ducks became sick, excreted the virus, and died. However, the ducks orally inoculated with 10⁴ 50% tissue culture infective doses of A/duck/Moscow/4182/2010 and challenged 14 to 90 days later with H5N1 HPAIV did not excrete the challenge virus. All contact chickens survived and did not excrete the virus. Our results suggest that low pathogenic virus of wild aquatic birds can be used for prevention of transmission of H5N1 viruses between ducks and chickens.
野禽已知能够携带禽流感病毒长途传播，并感染家鸭，而家鸭又会感染家鸡。因此，预防病毒在鸭和鸡之间的传播对于控制禽流感的传播非常重要。在这里，我们使用低致病性野生水禽病毒 A/duck/Moscow/4182/2010（H5N3）来预防高致病性禽流感病毒（HPAIV）在鸭和鸡之间的传播。我们首先证实，经口感染 H5N1 HPAIV A/chicken/Kurgan/3/2005 的鸭子会在粪便中排出病毒。所有与感染鸭子接触的鸡都会生病、排出病毒并死亡。然而，经口接种 10⁴ 50%组织培养感染剂量的 A/duck/Moscow/4182/2010，然后在 14 至 90 天后用 H5N1 HPAIV 进行攻毒的鸭子没有排出攻毒病毒。所有接触的鸡都存活下来，没有排出病毒。我们的结果表明，野生水禽的低致病性病毒可用于预防 H5N1 病毒在鸭和鸡之间的传播。
