School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, Bandar Sunway, 47500, Selangor, Darul Ehsan, Malaysia; School of Pharmacy and Applied Science, La Trobe Institute for Molecular Sciences, La Trobe University, Edwards Rd, Bendigo, 3550, Australia.
Sunway College, No. 2, Jalan Universiti, Bandar Sunway, 47500, Selangor, Darul Ehsan, Malaysia.
J Chromatogr A. 2018 May 18;1550:57-62. doi: 10.1016/j.chroma.2018.03.054. Epub 2018 Mar 28.
The interaction of bioactive compounds from ethanolic extracts of selected marine algae samples, separated on chromatographic plates, with nitric/nitrous acid was investigated. The nature of bioactive compounds in the marine algae extracts was characterised using UV absorption spectra before and after reaction with diluted nitric acid, and from the characteristic colour reaction after derivatization with anisaldehyde. It was found that diterpenes from Dictyota dichotoma, an edible brown algae, and sterols from green algae Caulerpa brachypus, bind nitric oxide and may act as a nitric oxide carrier. Although the carotenoid fucoxanthin, found in all brown marine algae also binds nitric oxide, the bonds between nitrogen and the fucoxanthin molecule are much stronger. Further studies are required to evaluate the effects of diterpenes from Dictyota dichotoma and sterols from green algae Caulerpa brachypus to see if they have beneficial cardiovascular effects. The method reported here should prove useful in screening large numbers of algae species for compounds with cardiovascular activity.
研究了在色谱板上分离的几种海洋藻类样品的醇提物中的生物活性化合物与硝酸/亚硝酸的相互作用。利用与稀硝酸反应前后的紫外吸收光谱以及用茴香醛衍生后的特征显色反应，对海洋藻类提取物中的生物活性化合物的性质进行了表征。结果表明，来自可食用褐藻双齿藻的二萜类化合物和绿藻泡叶藻中的甾醇可与一氧化氮结合，可能作为一氧化氮载体。虽然所有褐藻中都存在的类胡萝卜素岩藻黄素也能结合一氧化氮，但氮与岩藻黄素分子之间的键更强。需要进一步研究来评估来自双齿藻的二萜类化合物和绿藻泡叶藻中的甾醇是否具有有益的心血管作用。这里报道的方法应该有助于筛选具有心血管活性的大量藻类物种的化合物。
