Andrews S L, Evers W D
Department of Foods and Nutrition, Purdue University, West Lafayette, Indiana 47907.
Drug Nutr Interact. 1987;5(3):181-9.
Sprague-Dawley rats were raised by dams (mother reared, MR) or artificially reared from day 4 to day 11, using chronic intragastric cannulas, and infused with one of four diets: control (AR); or supplemented with nicotinamide-low (LN, 300 mg/l), medium (MN, 750 mg/l), or high (HN, 1500 mg/l). Liver to body weight ratios were higher in all artificially reared groups (AR, LN, MN, HN) compared to MR pups. The amount of recovered hepatic microsomal protein was lower in all artificially reared groups when compared with MR pups. Uridine diphosphoglucuronyl transferase activity with para-nitrophenol as the substrate (UDPGT-PNP) was greater in all of the artificially reared groups compared to the MR group. UDPGT-PNP activity in the HN group was greater than in the AR, LN, or MN groups. Cytochrome P-450 concentration was highest in the MR group, whereas there were no differences among the artificially reared groups. It was concluded that the artificial rearing process stimulated hepatic UDPGT-PNP activity and depressed cytochrome P-450 concentrations, whereas dietary supplementation with nicotinamide during the preweanling period resulted in a further increase in UDPGT-PNP activity.
将斯普拉格-道利大鼠从出生第4天至第11天由母鼠抚养（母养，MR）或人工饲养，采用慢性胃内插管法，并分别给予四种饮食之一：对照饮食（AR）；或添加低剂量烟酰胺（LN，300毫克/升）、中剂量烟酰胺（MN，750毫克/升）或高剂量烟酰胺（HN，1500毫克/升）。与母养幼崽相比，所有人工饲养组（AR、LN、MN、HN）的肝重与体重比更高。与母养幼崽相比，所有人工饲养组回收的肝微粒体蛋白量更低。以对硝基苯酚为底物的尿苷二磷酸葡萄糖醛酸基转移酶活性（UDPGT-PNP）在所有人工饲养组中均高于母养组。HN组的UDPGT-PNP活性高于AR、LN或MN组。细胞色素P-450浓度在母养组中最高，而人工饲养组之间无差异。研究得出结论，人工饲养过程刺激了肝脏UDPGT-PNP活性并降低了细胞色素P-450浓度，而在断奶前期间补充烟酰胺饮食导致UDPGT-PNP活性进一步增加。
