Wang Hongjin, Zhang Hongjun, Li Bin, Yu Zhihui, Li Guangrong, Zhang Jie, Yang Zujun
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
Center of Informational Biology, University of Electronic Science and Technology of China, Chengdu, China.
Front Plant Sci. 2018 Mar 19;9:365. doi: 10.3389/fpls.2018.00365. eCollection 2018.
As an important relative of wheat ( L), contains novel high molecular weight glutenin subunits (HMW-GSs) encoded by genes. We identified new wheat- chromosome introgression lines including chromosomes 1V and 1VL.5VL by fluorescence hybridization (FISH) combined with molecular markers. We found that chromosome changes occurred in the wheat- introgression lines and particularly induced the deletion of 5BS terminal repeats and formation of a new type of 5B-7B reciprocal translocation. The results imply that the chromosome 1V may contain genes which induce chromosomal recombination in wheat background. Ten putative high molecular weight glutenin subunit (HMW-GS) genes from and wheat- introgression lines were isolated. The lengths of the HMW-GS genes in were significantly shorter than typical HMW-GS of common wheat. A new y-type HMW-GS gene, named , was characterized in wheat- 1V introgression lines. The new wheat- germplasm displayed reduced plant height, increased tillers and superior grain protein and gluten contents, improved gluten performance index. The results showed considerable potential for utilization of chromosome 1V segments in future wheat improvement.
作为小麦（L）的一个重要近缘种，含有由基因编码的新型高分子量谷蛋白亚基（HMW - GSs）。我们通过荧光原位杂交（FISH）结合分子标记鉴定出包括1V染色体和1VL.5VL染色体的新型小麦 - 染色体渐渗系。我们发现小麦 - 渐渗系中发生了染色体变化，特别是诱导了5BS末端重复序列的缺失以及一种新型5B - 7B相互易位的形成。结果表明1V染色体可能含有在小麦背景中诱导染色体重组的基因。从小麦 - 渐渗系中分离出10个推定的高分子量谷蛋白亚基（HMW - GS）基因。中的HMW - GS基因长度明显短于普通小麦的典型HMW - GS。在小麦 - 1V渐渗系中鉴定出一个新的y型HMW - GS基因，命名为。新的小麦 - 种质表现出株高降低、分蘖增加、籽粒蛋白质和谷蛋白含量优异、谷蛋白性能指数提高。结果表明在未来小麦改良中利用1V染色体片段具有相当大的潜力。
