Fu Juan, Zeng Xianli, He Nongyue
J Nanosci Nanotechnol. 2017 Jan;17(1):196-202. doi: 10.1166/jnn.2017.12444.
Despite increasing biomedical applications of zinc oxide nanoparticles (ZnO NPs), there is a lack of information concerning the biological effects of ZnO NPs on human cells. The purpose of this study was to assess the comparative cytotoxicity in human prostate cells (PC-3 and RWPE-1) exposure to 20-nm ZnO NPs. Exposure to concentrations from 0 to 50 μg/mL of ZnO NPs reduced cell viability of PC-3 cells in a dose- and time-dependent manner; whereas it did not affect RWPE-1 cells. A dose-dependent increase in LDH leakage and intracellular reactive oxygen species was observed in PC-3 cells but not in RWPE-1 cells exposure to ZnO NPs at concentrations of 8 ˜ 50 μg/mL for 24 h (P < 0.05). That the percentage of apoptotic cells increased significantly was observed in PC-3 cells induced by ZnO NPs at 10 μg/mL exposure for 8 h. Our results showed that ZnO NPs induced in vitro preferential cytotoxicity in the human prostate cancer cells. We indicated that the different cytotoxicity of ZnO NPs is likely due to the different cell-nanoparticle interaction and response behavior rather than to hydrodynamic sizes of particles. It is suggested that ZnO NPs are expected to find a very promising targeting therapeutic application for human prostate cancer.
尽管氧化锌纳米颗粒（ZnO NPs）在生物医学领域的应用日益增加，但关于ZnO NPs对人类细胞生物学效应的信息却很匮乏。本研究的目的是评估20纳米ZnO NPs对人前列腺细胞（PC-3和RWPE-1）的比较细胞毒性。暴露于浓度为0至50μg/mL的ZnO NPs会以剂量和时间依赖性方式降低PC-3细胞的活力；而对RWPE-1细胞没有影响。在PC-3细胞中观察到，暴露于浓度为8至50μg/mL的ZnO NPs 24小时后，乳酸脱氢酶（LDH）泄漏和细胞内活性氧呈剂量依赖性增加，但在RWPE-1细胞中未观察到（P < 0.05）。在暴露于10μg/mL ZnO NPs 8小时的PC-3细胞中，观察到凋亡细胞百分比显著增加。我们的结果表明，ZnO NPs在体外对人前列腺癌细胞具有优先细胞毒性。我们指出，ZnO NPs不同的细胞毒性可能是由于细胞与纳米颗粒不同的相互作用和反应行为，而不是颗粒的流体动力学尺寸。有人提出，ZnO NPs有望在人类前列腺癌的靶向治疗应用中找到非常有前景的用途。
