Integrative Crop Ecophysiology Group, Plant Physiology Section, Faculty of Biology, University of Barcelona, Diagonal, Barcelona, Spain.
National Institute for Agricultural and Food Research and Technology (INIA), Madrid, Spain.
J Exp Bot. 2018 May 25;69(12):3081-3094. doi: 10.1093/jxb/ery109.
The effects of leaf dorsoventrality and its interaction with environmentally induced changes in the leaf spectral response are still poorly understood, particularly for isobilateral leaves. We investigated the spectral performance of 24 genotypes of field-grown durum wheat at two locations under both rainfed and irrigated conditions. Flag leaf reflectance spectra in the VIS-NIR-SWIR (visible-near-infrared-short-wave infrared) regions were recorded in the adaxial and abaxial leaf sides and at the canopy level, while traits providing information on water status and grain yield were evaluated. Moreover, leaf anatomical parameters were measured in a subset of five genotypes. The spectral traits studied were more affected by the leaf side than by the water regime. Leaf dorsoventral differences suggested higher accessory pigment content in the abaxial leaf side, while water regime differences were related to increased chlorophyll, nitrogen, and water contents in the leaves in the irrigated treatment. These variations were associated with anatomical changes. Additionally, leaf dorsoventral differences were less in the rainfed treatment, suggesting the existence of leaf-side-specific responses at the anatomical and biochemical level. Finally, the accuracy in yield prediction was enhanced when abaxial leaf spectra were employed. We concluded that the importance of dorsoventrality in spectral traits is paramount, even in isobilateral leaves.
叶片腹背性及其与叶片光谱响应的环境诱导变化之间的相互作用的影响仍知之甚少，特别是对于等面叶。我们在两个地点的雨养和灌溉条件下研究了 24 个田间生长的硬质小麦基因型的光谱性能。在 VIS-NIR-SWIR（可见-近红外-短波红外）区域记录了旗叶的正反两面和冠层水平的反射光谱，同时评估了提供水分状况和籽粒产量信息的性状。此外，在五个基因型的子集中测量了叶片解剖参数。研究的光谱特征受叶片侧的影响大于水分状况。叶片腹背差异表明，背面叶片的辅助色素含量更高，而水分状况的差异与叶片中叶绿素、氮和水分含量的增加有关，在灌溉处理中。这些变化与解剖变化有关。此外，在雨养处理中，叶片腹背差异较小，这表明在解剖学和生化水平上存在叶片特异性反应。最后，当使用背面叶片光谱时，提高了产量预测的准确性。我们得出的结论是，叶片腹背性在光谱特征中的重要性至关重要，即使对于等面叶也是如此。
