Gregory S H
Department of Basic Sciences, Marquette University School of Dentistry, Milwaukee, WI 53233.
J Leukoc Biol. 1988 Jan;43(1):67-79. doi: 10.1002/jlb.43.1.67.
Adherence to the culture substratum exerted a significant influence on the growth and survival of murine bone marrow cells incubated under conditions that favored the development of mononuclear phagocytes. Bone marrow cells cultured two weeks in unprocessed polystyrene dishes, refractory to cell attachment, reached a maximum or near maximum cell density by day 11. This density was stable throughout the duration of the incubation period. Characterization of the cells on day 11 revealed an essentially pure population of mononuclear phagocytes composed of round, nonadherent cells, and slightly elongated, loosely attached cells. Bone marrow cells incubated in polystyrene tissue culture dishes processed to promote cell attachment also reached a maximum cell density by day 11. However, this maximum density was only half that attained by cells incubated in unprocessed polystyrene dishes. Furthermore, continued incubation resulted in a sharp decline in cell viability and number. The cells in tissue culture dishes on day 11 represented a pure mononuclear phagocyte population composed principally of cells adherent to the surface of the dish. The subsequent analysis of cells subcultured in processed and unprocessed polystyrene dishes indicated that adherence to the substratum modulated the growth factor requirements of bone marrow-derived mononuclear phagocytes. Specifically, mononuclear phagocytes in tissue culture dishes expressed an elevated requirement for colony stimulating factor-1 in order to proliferate and survive in long-term culture.
对培养底物的黏附对在有利于单核吞噬细胞发育的条件下培养的小鼠骨髓细胞的生长和存活产生了重大影响。在未经处理的聚苯乙烯培养皿中培养两周的骨髓细胞，对细胞附着具有抗性，到第11天时达到最大或接近最大细胞密度。在整个培养期内，该密度保持稳定。对第11天的细胞进行表征发现，其基本上是由圆形、不黏附细胞以及略呈细长形、松散附着细胞组成的纯单核吞噬细胞群体。在经过处理以促进细胞附着的聚苯乙烯组织培养皿中培养的骨髓细胞到第11天时也达到了最大细胞密度。然而，这个最大密度仅为在未经处理的聚苯乙烯培养皿中培养的细胞所达到密度的一半。此外，继续培养会导致细胞活力和数量急剧下降。第11天时组织培养皿中的细胞代表了一个主要由附着在培养皿表面的细胞组成的纯单核吞噬细胞群体。随后对在经过处理和未经处理的聚苯乙烯培养皿中继代培养的细胞进行的分析表明，对底物的黏附调节了骨髓来源的单核吞噬细胞对生长因子的需求。具体而言，组织培养皿中的单核吞噬细胞为了在长期培养中增殖和存活，对集落刺激因子-1的需求增加。
