Hasan Md Kamrul, Alam Saruar, Mirkovic Jovan, Hossain Md Faruk
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh.
Department of Biological Sciences, St. John's University, Queens, New York 11439.
Bioinformation. 2018 Feb 28;14(2):68-74. doi: 10.6026/97320630014068. eCollection 2018.
Previous studies showed that prolonged exposure to fluoride (F-) and aluminum (Al3+) ions is associated with numerous diseases including neurological disorders. They don't have any known biological function. But they can bind with proteins that interact with ions similar to them. Such unwanted interactions affect the normal biological function of the target proteins, as well as their downstream protein-protein interactions. Several studies show the detrimental effects posed by them including Alzheimer's disease. However, their target proteins have never been reported. Here, we have screened for the human protein targets subjected to F- and Al3+ interactions by using data-driven prediction tools. We have identified 20 different proteins that directly bind with them (10 interact with fluoride and 10 with aluminum). In addition, protein-protein interaction has been explored to find the proteins that indirectly interact with F- and Al3+. We have found 86 indirect targets for F- and 90 for Al3+. Furthermore, 19 common protein targets have been identified, including proteins (9 out of 19) associated with neurodegenerative disorders. However, wet lab experiments are beyond our scopes to validate the binding networks. Additional studies must be warranted.
先前的研究表明，长期暴露于氟离子（F-）和铝离子（Al3+）与包括神经紊乱在内的多种疾病有关。它们没有任何已知的生物学功能。但它们可以与那些与它们类似的离子相互作用的蛋白质结合。这种不必要的相互作用会影响靶蛋白的正常生物学功能，以及它们下游的蛋白质-蛋白质相互作用。多项研究表明它们会造成有害影响，包括引发阿尔茨海默病。然而，它们的靶蛋白从未被报道过。在此，我们通过使用数据驱动的预测工具，筛选了与F-和Al3+相互作用的人类蛋白质靶标。我们鉴定出了20种与之直接结合的不同蛋白质（10种与氟化物相互作用，10种与铝相互作用）。此外，还探索了蛋白质-蛋白质相互作用，以找出与F-和Al3+间接相互作用的蛋白质。我们发现F-有86个间接靶标，Al3+有90个间接靶标。此外，还鉴定出了19个共同的蛋白质靶标，包括与神经退行性疾病相关的蛋白质（19个中的9个）。然而，通过湿实验室实验来验证这些结合网络超出了我们的能力范围。必须进行更多的研究。
