Racadot E, Herve P, Lamy B, Peters A
Centre Regional de Transfusion Sanguine, Besançon, France.
Leuk Res. 1987;11(11):987-94. doi: 10.1016/0145-2126(87)90117-2.
We analysed the optimal conditions for autologous bone marrow purging using complement binding monoclonal antibodies (mAbs). Twelve mAbs belonging to four clusters (CD9, CD10, CD19, CD24), alone or combined were evaluated by using direct cytotoxicity and clonogenic assays. We observed the following data: (1) optimal cytotoxicity was reached with doses of 1-10 micrograms mAbs for 10(7) cells, (2) the concentration of the cell suspension had to be below 3 X 10(7)/ml, (3) combinations of mAbs were more effective than a single mAb treatment, (4) in the case of an IgM isotype, there seems to be a clear dissociation between the amount needed for optimal toxicity and that for antigenic saturation measured by cytofluorometry.
我们分析了使用补体结合单克隆抗体（mAb）进行自体骨髓净化的最佳条件。通过直接细胞毒性和克隆形成试验评估了属于四个簇（CD9、CD10、CD19、CD24）的12种单克隆抗体，单独或联合使用。我们观察到以下数据：（1）对于10⁷个细胞，1-10微克单克隆抗体的剂量可达到最佳细胞毒性；（2）细胞悬液浓度必须低于3×10⁷/ml；（3）单克隆抗体联合使用比单一单克隆抗体治疗更有效；（4）在IgM同种型的情况下，最佳毒性所需量与通过细胞荧光测定法测量的抗原饱和所需量之间似乎存在明显的解离。
