Miao Sen, Wu Hao, Zhao Yue, Caiyin Qinggele, Li Yanni, Qiao Jianjun
Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.
Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, People's Republic of China.
Biotechnol Lett. 2018 Jun;40(6):941-948. doi: 10.1007/s10529-018-2550-3. Epub 2018 Apr 4.
To engineer a small nonconding RNA anti41 to enhance nisin yield by inhibiting the expression of glnR in Lactococcus lactis F44.
We constructed a screening library to determine appropriate artificial sRNAs and obtained a sRNA anti41 that can produce approximately three fold of the inhibitory effect on GlnR. Moreover, the transcription levels of the direct inhibitory targets of GlnR (glnP, glnQ, amtB, and glnK) were dramatically upregulated in the anti41 overexpression strain (F44-anti41), thereby confirming the inhibitory effect of anti41 on GlnR. In addition, anti41 overexpression improved the survival rate of cells by approximately three fold under acid stress, promoted cell growth, and increased nisin yield by 29.83%.
We were able to provide a novel strategy for the construction of robust high-producing industrial strains.
构建一种小的非编码RNA（anti41），通过抑制乳酸乳球菌F44中glnR的表达来提高乳酸链球菌素的产量。
我们构建了一个筛选文库来确定合适的人工小RNA，并获得了一种sRNA（anti41），它对GlnR能产生约三倍的抑制作用。此外，GlnR的直接抑制靶点（glnP、glnQ、amtB和glnK）的转录水平在anti41过表达菌株（F44-anti41）中显著上调，从而证实了anti41对GlnR的抑制作用。此外，anti41过表达在酸胁迫下使细胞存活率提高了约三倍，促进了细胞生长，并使乳酸链球菌素产量提高了29.83%。
我们能够为构建强大的高产工业菌株提供一种新策略。
