Fcγ受体对抗免疫球蛋白诱导小鼠B淋巴细胞中c-myc mRNA表达的影响。
Fc gamma receptor effects on induction of c-myc mRNA expression in mouse B lymphocytes by anti-immunoglobulin.
作者信息
Phillips N E, Parker D C
机构信息
Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester 01605.
出版信息
Mol Immunol. 1987 Nov;24(11):1199-205. doi: 10.1016/0161-5890(87)90166-0.
Levels of c-myc mRNA have been assayed in mouse B cells cultured for 8 hr with Fab'2 anti-Ig or IgG anti-Ig. Fab'2 anti-Ig induces DNA synthesis in B cells, whereas the whole molecule inhibits anti-Ig-induced DNA synthesis by crosslinking the B cell Fc gamma R to mIg. Both the Fab'2 fragment and the IgG anti-Ig induce an increase in c-myc mRNA by 1 hr. Thereafter, levels in cells stimulated with submitogenic doses of Fab'2 anti-Ig or any dose of IgG anti-Ig returned to background, while levels in cultures containing a mitogenic dose of Fab'2 anti-Ig remained elevated.
用Fab'2抗Ig或IgG抗Ig培养8小时的小鼠B细胞中，已检测了c-myc mRNA的水平。Fab'2抗Ig可诱导B细胞中的DNA合成，而完整分子通过将B细胞FcγR与mIg交联来抑制抗Ig诱导的DNA合成。Fab'2片段和IgG抗Ig均可在1小时内诱导c-myc mRNA增加。此后，用亚致有丝分裂剂量的Fab'2抗Ig或任何剂量的IgG抗Ig刺激的细胞中的水平恢复到背景水平，而含有促有丝分裂剂量的Fab'2抗Ig的培养物中的水平仍保持升高。
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