Institute of Respiratory Disease, Xinqiao Hospital of Third Military Medical University, Chongqing 400037, P.R. China.
Mol Med Rep. 2018 Jun;17(6):7477-7486. doi: 10.3892/mmr.2018.8799. Epub 2018 Mar 28.
Streptococcus mitis (S. mitis) may transform into highly pathogenic bacteria. The aim of the present study was to identify potential antigen targets for designing an effective vaccine against the pathogenic S. mitis321A. The genome of S. mitis321A was sequenced using an Illumina Hiseq2000 instrument. Subsequently, Glimmer 3.02 and Tandem Repeat Finder (TRF) 4.04 were used to predict genes and tandem repeats, respectively, with DNA sequence function analysis using the Basic Local Alignment Search Tool (BLAST) in the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of Orthologous Groups of proteins (COG) databases. Putative gene antigen candidates were screened with BLAST ahead of phylogenetic tree analysis. The DNA sequence assembly size was 2,110,680 bp with 40.12% GC, 6 scaffolds and 9 contig. Consequently, 1,944 genes were predicted, and 119 TRF, 56 microsatellite DNA, 10 minisatellite DNA and 154 transposons were acquired. The predicted genes were associated with various pathways and functions concerning membrane transport and energy metabolism. Multiple putative genes encoding surface proteins, secreted proteins and virulence factors, as well as essential genes were determined. The majority of essential genes belonged to a phylogenetic lineage, while 321AGL000129 and 321AGL000299 were on the same branch. The current study provided useful information regarding the biological function of the S. mitis321A genome and recommends putative antigen candidates for developing a potent vaccine against S. mitis.
表型温和链球菌（Streptococcus mitis，S. mitis）可能会转化为高致病性细菌。本研究旨在鉴定潜在的抗原靶标，以设计针对致病性 S. mitis321A 的有效疫苗。使用 Illumina Hiseq2000 仪器对 S. mitis321A 的基因组进行测序。随后，分别使用 Glimmer 3.02 和 Tandem Repeat Finder（TRF）4.04 预测基因和串联重复序列，使用基本局部比对搜索工具（BLAST）在京都基因与基因组百科全书（KEGG）和蛋白质直系同源群（COG）数据库中对 DNA 序列功能进行分析。在进行系统发育树分析之前，使用 BLAST 对假定的基因抗原候选物进行筛选。DNA 序列组装大小为 2110680bp，GC 含量为 40.12%，有 6 个支架和 9 个连续体。因此，预测到 1944 个基因，获得了 119 个串联重复序列、56 个微卫星 DNA、10 个小卫星 DNA 和 154 个转座子。预测的基因与涉及膜转运和能量代谢的各种途径和功能相关。确定了多个编码表面蛋白、分泌蛋白和毒力因子以及必需基因的假定基因。大多数必需基因属于一个系统发育谱系，而 321AGL000129 和 321AGL000299 位于同一分支上。本研究为 S. mitis321A 基因组的生物学功能提供了有用的信息，并推荐了潜在的抗原候选物，以开发针对 S. mitis 的有效疫苗。
