College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China; State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing 210095, China.
Food Chem. 2018 Aug 15;257:289-294. doi: 10.1016/j.foodchem.2018.02.148. Epub 2018 Mar 2.
An upconversion fluorescence DNA probe which consists of aptamer-conjugated magnet nanoparticles (apt-MNPs) and complementary DNA-conjugated upconversion nanoparticles (cDNA-UCNPs) was developed to detect acetamiprid. Acetamiprid can specifically conjugate with the apt-MNPs to dissociate the cDNA-UCNPs from the apt-MNPs and resulted in reduced fluorescence intensity through an external magnet. The change of fluorescence intensity (△I) is positively related to the concentration of acetamiprid, which can be applied for the quantification of acetamiprid. Under optimal conditions, a linear detection range and detection limit are 0.89-114.18 μg/L and 0.65 μg/L, respectively. The probe was successfully used to detect acetamiprid in spiked paddy water, soil, pear, apple, wheat and cucumber. Average recoveries are 78.2%-103.5% with intra-day relative standard deviations (RSDs) of 2.6%-10.9% and inter-day RSDs of 4.3%-10.2%. The amounts of acetamiprid in the authentic paddy water and pear samples detected by the DNA probe are significantly correlated with that detected by high-performance liquid chromatography (HPLC).
一种上转换荧光 DNA 探针，由适配体偶联磁纳米粒子（apt-MNPs）和互补 DNA 偶联上转换纳米粒子（cDNA-UCNPs）组成，用于检测吡虫啉。吡虫啉可以特异性地与 apt-MNPs 结合，使 cDNA-UCNPs 从 apt-MNPs 上解离，并通过外部磁铁导致荧光强度降低。荧光强度的变化（△I）与吡虫啉的浓度呈正相关，可用于吡虫啉的定量检测。在最佳条件下，线性检测范围和检测限分别为 0.89-114.18μg/L 和 0.65μg/L。该探针成功用于检测添加到稻田水中、土壤、梨、苹果、小麦和黄瓜中的吡虫啉。平均回收率为 78.2%-103.5%，日内相对标准偏差（RSD）为 2.6%-10.9%，日间 RSD 为 4.3%-10.2%。用 DNA 探针检测到的真实稻田水和梨样品中的吡虫啉含量与高效液相色谱（HPLC）检测到的吡虫啉含量有显著相关性。
