Department of Biology, College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China.
Department of Biology, College of Marine Life Sciences, Ocean University of China, Yushan Road, No. 5, Qingdao, China; Department of Biology, Shantou University, Shantou 515063, China.
Food Chem. 2018 Aug 15;257:29-35. doi: 10.1016/j.foodchem.2018.03.003. Epub 2018 Mar 2.
A very high pullulan producing yeast-like fungus, Aureobasidium melanogenum TN1-2 isolated from a natural honey was found to be able to produce 97.0 g/L of pullulan from 140.0 g/L sucrose at a flask level while it could yield 114.0 g/L of pullulan within 134 h during a 10-liter fermentation, the yield was 0.81 g/g and the productivity was 0.86 g/L/h. The high ability to biosynthesize pullulan by this yeast-like fungal strain TN1-2 was related to high glucosyltransferase activity, high phosphofructo-2-kinase activity, high content of its cellular glycerol and low glucose repressor. The Mw of the produced pullulan was 1.42 × 10 g/mol. The low Mw may be due to the high α-amylase, glucoamylase and isopullulanase activities. The intracellular level of trehalose had no influence on high pullulan production by the yeast-like fungal strain TN1-2.
从天然蜂蜜中分离到一株产超高普鲁兰的酵母样真菌，即 melanogenum TN1-2，在摇瓶水平上，该菌能以 140.0 g/L 的蔗糖生产 97.0 g/L 的普鲁兰，在 10 升发酵罐中 134 h 内可生产 114.0 g/L 的普鲁兰，产率为 0.81 g/g，生产强度为 0.86 g/L/h。该酵母样真菌 TN1-2 具有很强的合成普鲁兰的能力，这与其具有较高的葡萄糖基转移酶活性、较高的磷酸果糖-2-激酶活性、较高的细胞甘油含量和较低的葡萄糖阻遏物有关。所产生的普鲁兰的 Mw 为 1.42×10 g/mol。Mw 较低可能是由于α-淀粉酶、糖化酶和异普鲁兰酶活性较高所致。胞内海藻糖水平对酵母样真菌 TN1-2 高产普鲁兰没有影响。
