Rahman Khaliqur, Gupta Ruchi, Harankhedkar Shivangi, Gupta Tanvi, Sarkar Manoj K, Nityanand Soniya
Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGIMS), Lucknow, 226014 India.
Indian J Hematol Blood Transfus. 2018 Apr;34(2):304-309. doi: 10.1007/s12288-017-0867-z. Epub 2017 Aug 22.
To study the utility and advantage of CD157 in the paroxysmal nocturnal hemoglobinuria (PNH) screening along with its ability to replace CD24 and CD14. This was a confirmatory study to analyse the role and advantage of CD157 in a single tube five color combination to identify the PNH clones. A serial tenfold dilution experiments was carried out for sensitivity assessment. Reproducibility was checked in the intra-assay and inter-assay experiments. The results obtained with CD157 based assay were compared with the routinely used single tube six color CD24/CD14 based assay. CD157 showed a high degree of sensitivity at the level of 10. PNH positive clone sizes were precise with CVs of inter-assay and intra-assay precision analysis for polymorphs/monocytes ranging from 2.94 to 4.31/2.52 to 8.93, and 0.91 to 3.23/1.65 to 5.33%; respectively. The results were similar to those obtained from CD24/CD14 based assay (R > 0.993). There was no false positive or false negative result. CD157 was found better in delineating the type II clones. CD157 can be used as a common PNH leucocyte marker with high degree of sensitivity and precision. It can replace CD24 and CD14 from the currently used assays and thus bring down the cost of PNH screening.
研究CD157在阵发性夜间血红蛋白尿（PNH）筛查中的效用和优势，以及其替代CD24和CD14的能力。这是一项验证性研究，旨在分析CD157在单管五色组合中识别PNH克隆的作用和优势。进行了系列十倍稀释实验以评估灵敏度。在批内和批间实验中检查了重复性。将基于CD157的检测结果与常规使用的基于单管六色CD24/CD14的检测结果进行比较。CD157在10的水平上显示出高度的灵敏度。PNH阳性克隆大小精确，多形核白细胞/单核细胞的批间和批内精密度分析的变异系数分别为2.94%至4.31%/2.52%至8.93%，以及0.91%至3.23%/1.65%至5.33%。结果与基于CD24/CD14的检测结果相似（R>0.993）。没有假阳性或假阴性结果。发现CD157在区分II型克隆方面表现更好。CD157可作为一种具有高度灵敏度和精密度的常见PNH白细胞标志物。它可以替代目前检测方法中的CD24和CD14，从而降低PNH筛查的成本。
