关于醛固定后组织块内肌原纤维ATP酶活性分布的组织光度测量。
Histophotometrical measurements concerning the distribution of myofibrillar ATPase activity within the tissue block after aldehyde fixation.
作者信息
Punkt K, Krug H, Böhme R
机构信息
Department of Quantitative Morphology, Karl Marx University Leipzig, GDR.
出版信息
Acta Histochem. 1987;82(1):109-13. doi: 10.1016/S0065-1281(87)80061-2.
The inhibition of myofibrillar ATPase activity by aldehyde fixation in tissue pieces of myocardium of the rat was measured by scanning histophotometry. The relative amount of the final reaction product of the histochemical method for ATPase (Padykula and Herman 1955) was taken as a measure for the enzyme activity. Along scanning lines in slides from the surface to the centre of tissue block, a higher activity was found in the centre in contrast to the marginal zone. Compared with the centre of the block (approximately equal to 100%), in the marginal region a loss of about 40% (in paraformaldehyde PFA) and of 75% (in glutaraldehyde GA) was found, the latter with a sharp decline between the marginal zone and the central part. For quantitative enzyme histochemistry by histophotometry unfixed material is recommended.
通过扫描组织光度测定法测量醛固定对大鼠心肌组织块中肌原纤维ATP酶活性的抑制作用。ATP酶组织化学方法（Padykula和Herman，1955年）的最终反应产物的相对量被用作酶活性的指标。在从组织块表面到中心的载玻片上沿扫描线观察，发现中心的活性高于边缘区域。与组织块中心（约等于100%）相比，边缘区域的活性损失约为40%（在多聚甲醛PFA中）和75%（在戊二醛GA中），后者在边缘区域和中心部分之间急剧下降。对于通过组织光度测定法进行的定量酶组织化学，建议使用未固定的材料。
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