Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhopal, 462066, India.
Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal, Bhopal Bypass Road, Bhopal, 462066, India.
Chembiochem. 2018 Jul 4;19(13):1386-1390. doi: 10.1002/cbic.201800134. Epub 2018 May 24.
A small molecule, perylene bisimide imidazolyl derivative (PBI-ID), has been identified and developed as a specific marker for labelling multifunctional fat bodies in various organisms, including Drosophila and mammalian adipocytes. Interestingly, PBI-ID neither labels the plasma membranes nor cell nuclei by trapping into it. A remarkable feature of unbound PBI-ID is diminished fluorescence, which reduces the background emission noise, while contrasting the bound state effectively.
一种小分子，即苝二酰亚胺咪唑基衍生物（PBI-ID），已被鉴定和开发为标记各种生物体多功能脂肪体的特定标志物，包括果蝇和哺乳动物脂肪细胞。有趣的是，PBI-ID 既不通过捕获进入其中来标记质膜也不标记细胞核。未结合的 PBI-ID 的一个显著特征是荧光减弱，这降低了背景发射噪声，同时有效地对比了结合状态。
