Bioinformatics Centre, Institute of Microbial Technology, Council of Scientific and Industrial Research, Sector 39A, Chandigarh 160036, India.
Bioinformatics Centre, Institute of Microbial Technology, Council of Scientific and Industrial Research, Sector 39A, Chandigarh 160036, India.
J Mol Biol. 2018 Jul 20;430(15):2212-2218. doi: 10.1016/j.jmb.2018.03.023. Epub 2018 Apr 3.
RNA activation (RNAa) is the process of enhancing selective gene expression at transcriptional level using double-stranded RNAs, targeting gene promoter. These RNA molecules are usually 21 nucleotides long and termed as small activating RNAs (saRNAs). They are involved in gene regulation, epigenetics, gain-of-function studies and have potential therapeutic applications for various diseases especially cancer. RNAa is opposite to RNA interference in functionality; however, both processes share some protein machinery. There are many RNA interference centered online resources but no one for saRNAs; therefore, we developed "saRNAdb" database (http://bioinfo.imtech.res.in/manojk/sarna/). It contains 2150 manually curated saRNA entries with detailed information about their nucleotide sequences, activities, corresponding target gene, promoter and other experimental data. Besides, saRNA-promoter binding location, predicted saRNA features, tools (off-target, map) and RNAa-related proteins with their interacting partners are provided. saRNAdb is expected to assist in RNA research especially for nucleic acid-based therapeutics development.
RNA 激活（RNAa）是一种利用双链 RNA 在转录水平上增强选择性基因表达的过程，其靶向基因启动子。这些 RNA 分子通常长 21 个核苷酸，被称为小激活 RNA（saRNA）。它们参与基因调控、表观遗传学、功能获得研究，并具有治疗各种疾病（特别是癌症）的潜在应用。RNAa 在功能上与 RNA 干扰相反；然而，这两个过程共享一些蛋白质机制。有许多以 RNA 干扰为中心的在线资源，但没有一个是针对 saRNA 的；因此，我们开发了“saRNAdb”数据库（http://bioinfo.imtech.res.in/manojk/sarna/）。它包含 2150 条经过人工精心整理的 saRNA 条目，提供了有关其核苷酸序列、活性、相应靶基因、启动子和其他实验数据的详细信息。此外，还提供了 saRNA-启动子结合位置、预测的 saRNA 特征、工具（脱靶、图谱）以及与 RNAa 相关的蛋白质及其相互作用伙伴。saRNAdb 有望为 RNA 研究提供帮助，特别是在核酸治疗药物开发方面。
