NGS 结合系统进化分析检测罗马尼亚静脉注射吸毒人群中的 HIV-1 双重感染。

NGS combined with phylogenetic analysis to detect HIV-1 dual infection in Romanian people who inject drugs.

机构信息

University of Medicine and Pharmacy "Carol Davila", Bucharest, Romania.

Molecular Diagnostics Laboratory, National Institute for Infectious Diseases "Matei Bals", Bucharest, Romania.

出版信息

Microbes Infect. 2018 May;20(5):308-311. doi: 10.1016/j.micinf.2018.03.004. Epub 2018 Apr 4.

DOI:10.1016/j.micinf.2018.03.004

PMID:29626632

Abstract

Dual HIV infections are possible and likely in people who inject drugs (PWID). Thirty-eight newly diagnosed patients, 19 PWID and 19 heterosexually HIV infected were analyzed. V2V3 loop of HIV-1 env gene was sequenced on the NGS platform 454 GSJunior (Roche). HIV-1 dual/multiple infections were identified in five PWID. For three of these patients, the reconstructed variants belonged to pure F1 subtype and CRF14_BG strains according to phylogenetic analysis. New recombinant forms between these parental strains were identified in two PWID samples. NGS data can provide, with the help of phylogenetic analysis, important insights about the intra-host sub-population structure.

摘要

双重 HIV 感染在注射毒品的人群（PWID）中是可能且常见的。对 38 名新诊断出的患者进行了分析，其中 19 名是 PWID，19 名是异性恋 HIV 感染者。在 NGS 平台 454GSJunior（罗氏）上对 HIV-1 env 基因的 V2V3 环进行了测序。在 5 名 PWID 中发现了 HIV-1 双重/多重感染。对其中 3 名患者进行系统进化分析，发现重建的变异体属于纯 F1 亚型和 CRF14_BG 株。在 2 名 PWID 样本中发现了这些亲本株之间的新重组形式。借助系统进化分析，NGS 数据可以提供有关宿主内亚种群结构的重要见解。