Universidad Nacional de Colombia, Sede Bogotá, Facultad de Ciencias, Departamento de Farmacia, FaMeTra research group (traditional and Popular medicine Pharmacology), Carrera 30 45-03, Bogotá D.C. 111311, Colombia.
Huitoto representative, Km 7 Via Leticia Tarapaca, Comunidad Ciudad Hitoma, Leticia, Colombia.
J Ethnopharmacol. 2018 Aug 10;222:288-294. doi: 10.1016/j.jep.2018.04.008. Epub 2018 Apr 4.
In the Leticia-Amazonas area, Uitoto indigenous people use a preparation of Curarea toxicofera (Wedd) Barneby & Krukoff (Menispermaceae) alone or combined with prescribed medications to prevent and treat malaria.
To determine the in vitro and in vivo antiplasmodial activity of traditional preparations of Curarea toxicofera alone and in combination with classical antimalarials.
The traditional preparation was evaluated in vitro against P. falciparum FCR3 CQ resistant strain, alone and combined. The preparation was further administered orally alone or combined with chloroquine and artesunate in mice infected with Plasmodium berghei ANKA strain on the four-day antimalarial test model.
The herbal remedy used alone was able to significantly decrease the parasitemia both in vitro (IC 7.3 µg/ml) and in vivo (ED 328 mg/Kg) but it was less active than chloroquine (IC 0.29 µg/ml in vitro and ED 2.3 mg/Kg/day in vivo), and than artesunate (IC 0.002 µg/ml and ED 3.7 mg/Kg/day). Interestingly it presented synergism with chloroquine in vitro (Combination Index: 0.39) and in vivo; and was additive with artesunate in vitro (Combination Index: 0.94) and in vivo.
The traditional preparation showed potential as an antimalarial and, when used in combination, does not negatively affect the efficacy of the drugs evaluated. Pre-clinical studies should be conducted with a standardized preparation to confirm its efficacy and safety alone and in combination with chloroquine and artesunate.
在莱蒂西亚-亚马逊地区，乌图托土著人民使用一种由毒芹 Curarea toxicofera（Wedd）Barneby & Krukoff（防己科）制成的制剂，单独或与规定药物联合使用，以预防和治疗疟疾。
确定毒芹 Curarea toxicofera 的传统制剂单独使用和与经典抗疟药物联合使用的体外和体内抗疟活性。
评估传统制剂对 P. falciparum FCR3 CQ 耐药株的体外活性，单独和联合使用。该制剂进一步在感染 Plasmodium berghei ANKA 株的小鼠中进行口服给药，单独或与氯喹和青蒿琥酯联合使用，在四天抗疟试验模型中。
单独使用的草药疗法能够显著降低体外（IC 7.3µg/ml）和体内（ED 328mg/Kg）的寄生虫血症，但它的活性低于氯喹（IC 0.29µg/ml 体外和 ED 2.3mg/Kg/天体内）和青蒿琥酯（IC 0.002µg/ml 和 ED 3.7mg/Kg/天）。有趣的是，它在体外（联合指数：0.39）和体内与氯喹表现出协同作用；并且在体外（联合指数：0.94）和体内与青蒿琥酯呈相加作用。
传统制剂显示出作为抗疟药的潜力，并且在联合使用时不会对评估药物的疗效产生负面影响。应使用标准化制剂进行临床前研究，以单独和与氯喹和青蒿琥酯联合使用来确认其疗效和安全性。
