Heeg K, Steeg C, Wagner H
Department of Medical Microbiology and Immunology, University of Ulm, West Germany.
Cell Immunol. 1988 Jan;111(1):148-57. doi: 10.1016/0008-8749(88)90059-7.
The aim of this study was to analyze in vivo the L3T4+ T-cell-subset-independent reactivity of Lyt2+ T cells toward transplantation alloantigens. To this end, we depleted normal mice of L3T4+ T cells by injection of monoclonal antibodies to the L3T4 antigen. This procedure not only led phenotypically to a disappearance of L3T4+ T cells, but also effectively abolished reactivity toward class II MHC antigens in vitro and in vivo. However, L3T4+ T-cell-depleted mice still reacted to class I MHC alloantigens in vivo: after immunization with class I MHC alloantigens Il-2 receptor-bearing T cells appeared in the draining lymph nodes, and developed antigen-specific cytolytic activity. Moreover, upon in vivo priming the frequencies of class I MHC-specific precursors of Il-2-producing and cytolytic Lyt2+ T lymphocytes increased up to 20-fold. L3T4+ T-cell-depleted mice rejected class I MHC-bearing skin grafts promptly. We conclude that not only in vitro but also in vivo Lyt2+ T cells remain reactive toward class I MHC antigens in the absence of L3T4+ T helper cells.
本研究的目的是在体内分析Lyt2⁺ T细胞对移植同种异体抗原的不依赖L3T4⁺ T细胞亚群的反应性。为此，我们通过注射针对L3T4抗原的单克隆抗体来清除正常小鼠体内的L3T4⁺ T细胞。这一过程不仅在表型上导致L3T4⁺ T细胞消失，而且在体外和体内均有效地消除了对II类MHC抗原的反应性。然而，L3T4⁺ T细胞耗竭的小鼠在体内仍对I类MHC同种异体抗原产生反应：在用I类MHC同种异体抗原免疫后，引流淋巴结中出现了携带IL-2受体的T细胞，并产生了抗原特异性细胞溶解活性。此外，在体内启动后，产生IL-2的和具有细胞溶解活性的Lyt2⁺ T淋巴细胞的I类MHC特异性前体细胞频率增加了20倍。L3T4⁺ T细胞耗竭的小鼠迅速排斥了携带I类MHC的皮肤移植物。我们得出结论，不仅在体外，而且在体内，在没有L3T4⁺ T辅助细胞的情况下，Lyt2⁺ T细胞仍对I类MHC抗原具有反应性。
