福尔马林固定、石蜡包埋组织样本的生物医学分析：分子诊断的圣杯。
Biomedical analysis of formalin-fixed, paraffin-embedded tissue samples: The Holy Grail for molecular diagnostics.
机构信息
Horváth Csaba Laboratory of Bioseparation Sciences, Research Centre for Molecular Medicine, Faculty of Medicine, University of Debrecen, 4032, Debrecen, Nagyerdei krt 98, Hungary.
Horváth Csaba Laboratory of Bioseparation Sciences, Research Centre for Molecular Medicine, Faculty of Medicine, University of Debrecen, 4032, Debrecen, Nagyerdei krt 98, Hungary; MTA-PE Translational Glycomics Research Group, Research Institute for Biomolecular and Chemical Engineering, University of Pannonia, Veszprem, Hungary.
出版信息
J Pharm Biomed Anal. 2018 Jun 5;155:125-134. doi: 10.1016/j.jpba.2018.03.065. Epub 2018 Apr 2.
More than a century ago in 1893, a revolutionary idea about fixing biological tissue specimens was introduced by Ferdinand Blum, a German physician. Since then, a plethora of fixation methods have been investigated and used. Formalin fixation with paraffin embedment became the most widely used types of fixation and preservation method, due to its proper architectural conservation of tissue structures and cellular shape. The huge collection of formalin-fixed, paraffin-embedded (FFPE) sample archives worldwide holds a large amount of unearthed information about diseases that could be the Holy Grail in contemporary biomarker research utilizing analytical omics based molecular diagnostics. The aim of this review is to critically evaluate the omics options for FFPE tissue sample analysis in the molecular diagnostics field.
一个多世纪前的 1893 年，德国医生费迪南德·布鲁姆（Ferdinand Blum）提出了一项关于固定生物组织标本的革命性想法。从那时起，人们研究并使用了大量的固定方法。由于福尔马林固定石蜡包埋（FFPE）能够很好地保存组织结构和细胞形态，因此成为最广泛使用的固定和保存方法。世界各地大量的福尔马林固定石蜡包埋（FFPE）样本档案库中保存着大量有关疾病的信息，这些信息可能是利用基于分析组学的分子诊断进行当代生物标志物研究的圣杯。本文旨在批判性地评估分子诊断领域中 FFPE 组织样本分析的组学选择。
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