Department of Microbiology, University of Agriculture in Cracow, Mickiewicza Ave 24/28, 30-059, Cracow, Poland.
Environ Monit Assess. 2018 Apr 7;190(5):269. doi: 10.1007/s10661-018-6644-0.
The aim of this study was to assess the microbiological quality of air in three horse riding centers differing in the horse keeping systems. The air samples were collected in one facility with free-range horse keeping system and two with box stalls of different sizes. The samples were collected over a period of 3 years (2015-2017), four times per year (spring, summer, autumn, winter) to assess the effect of seasonal changes. The prevalence of aerobic mesophilic bacteria, mold fungi, actinomycetes, Staphylococcus spp., and Escherichia coli was determined by the air collision method on Petri dishes with appropriate microbiological media. At the same time, air temperature, relative humidity, and particulate matter concentration (PM, PM) were measured. It was found that the horse keeping system affects the occurrence of the examined airborne microorganisms. Over the 3-year period of study, higher temperature and humidity, as well as particulate matter concentration-which notoriously exceeded limit values-were observed in the facilities with the box-stall system. The air sampled from the largest horse riding center, with the largest number of horses and the box-stall system of horse keeping, was also characterized by the heaviest microbiological contamination. Among others, bacteria from the following genera: Staphylococcus spp., Streptococcus spp., Bacillus spp., and E. coli and fungi from the genera Aspergillus, Fusarium, Mucor, Rhizopus, Penicillium, Trichothecium, Cladosporium, and Alternaria were identified in the analyzed samples.
本研究旨在评估三种骑马中心空气中的微生物质量，这三种骑马中心的马匹饲养系统不同。在一个采用自由放养马饲养系统的设施中以及两个采用不同大小马厩的设施中收集空气样本。在 3 年（2015-2017 年）的时间内，每年收集四次（春季、夏季、秋季和冬季）样本，以评估季节性变化的影响。通过在带有适当微生物培养基的培养皿上使用空气撞击法来确定需氧嗜温细菌、霉菌真菌、放线菌、金黄色葡萄球菌和大肠杆菌的流行率。同时，测量空气温度、相对湿度和颗粒物浓度（PM、PM）。研究发现，马匹饲养系统会影响所检查的空气传播微生物的发生。在为期 3 年的研究中，在采用马厩系统的设施中，观察到更高的温度和湿度以及颗粒物浓度（众所周知，颗粒物浓度超过了限值）。从马匹数量最多且采用马厩系统的最大的骑马中心采集的空气样本也具有最严重的微生物污染特征。在分析样本中鉴定出以下属的细菌：金黄色葡萄球菌、链球菌属、芽孢杆菌属和大肠杆菌，以及属曲霉菌、镰刀菌属、毛霉属、根霉属、青霉属、木霉属、枝孢属、链格孢属和交替单胞菌属的真菌。
