补充鱼油可缓解高脂饮食喂养大鼠血液、肝脏和脂肪组织中脂质稳态的改变。
Fish Oil Supplementation Alleviates the Altered Lipid Homeostasis in Blood, Liver, and Adipose Tissues in High-Fat Diet-Fed Rats.
作者信息
Chiu Chen-Yuan, Wang Lou-Pin, Liu Shing-Hwa, Chiang Meng-Tsan
机构信息
Institute of Food Safety and Health, College of Public Health , National Taiwan University , Taipei 100 , Taiwan.
Department of Food Science, College of Life Science , National Taiwan Ocean University , Keelung 202 , Taiwan.
出版信息
J Agric Food Chem. 2018 Apr 25;66(16):4118-4128. doi: 10.1021/acs.jafc.8b00529. Epub 2018 Apr 16.
This study investigated the effects of dietary supplementation of fish oil on the signals of lipid metabolism involved in hepatic cholesterol and triglyceride influx and excretion in high-fat diet (HFD)-fed rats. Fish oil (FO) repressed body (HFD, 533 ± 18.2 g; HFD+FO, 488 ± 28.0 g, p < 0.05) and liver weights (HFD, 5.7 ± 0.6 g/100 g of body weight; HFD+FO, 4.8 ± 0.4 g/100 g of body weight, p < 0.05) in HFD-fed rats. Fish oil could also improve HFD-induced imbalance of lipid metabolism in blood, liver, and adipose tissues including the significant decreases in plasma and liver total cholesterol (TC) (plasma-HFD, 113 ± 33.6 mg/dL; HFD+FO, 50.0 ± 5.95 mg/dL, p < 0.05; liver-HFD, 102 ± 13.0 mg/g liver; [corrected] HFD+FO, 86.6 ± 7.81 mg/g liver, [corrected] p < 0.05), blood, liver, and adipose triglyceride (TG) (blood-HFD, 52.5 ± 20.4 mg/dL; HFD+FO, 29.8 ± 4.30 mg/dL, p < 0.05; liver-HFD, 56.2 ± 10.0 mg/g liver; [corrected] HFD+FO, 30.3 ± 5.28 mg/g liver, [corrected] p < 0.05; adipose-HFD, 614 ± 73.2 mg/g liver, [corrected] HFD+FO, 409 ± 334 mg/g of adipose tissue, [corrected] p < 0.05), and low density (HFD, 79.8 ± 40.9 mg/dL; HFD+FO, 16.6 ± 5.47 mg/dL, p < 0.05) and very-low-density (HFD, 49.7 ± 33.3 mg/dL; HFD+FO, 10.4 ± 3.45 mg/dL, p < 0.05) lipoprotein and the significant increases in fecal TC (HFD, 12.2 ± 0.67 mg/g feces; [corrected] HFD+FO, 16.3 ± 2.04 mg/g feces, [corrected] < 0.05) and TG (HFD, 2.09 ± 0.10 mg/g feces; [corrected] HFD+FO, 2.38 ± 0.22 mg/g feces, [corrected] p < 0.05) and lipoprotein lipase activity of adipose tissues (HFD, 16.6 ± 3.64 μM p-nitrophenol; HFD+FO, 24.5 ± 4.19 μM p-nitrophenol, p < 0.05). Moreover, fish oil significantly activated the protein expressions of hepatic lipid metabolism regulators (AMPKα and PPARα) and significantly regulated the lipid-transport-related signaling molecules (ApoE, MTTP, ApoB, Angptl4, ApoCIII, ACOX1, and SREBPF1) in blood or liver of HFD-fed rats. These results suggest that fish oil supplementation improves HFD-induced imbalance of lipid homeostasis in blood, liver, and adipose tissues in rats.
本研究调查了高脂饮食（HFD）喂养的大鼠中，膳食补充鱼油对参与肝脏胆固醇和甘油三酯流入及排泄的脂质代谢信号的影响。鱼油抑制了HFD喂养大鼠的体重（HFD组，533±18.2克；HFD+FO组，488±28.0克，p<0.05）和肝脏重量（HFD组，5.7±0.6克/100克体重；HFD+FO组，4.8±0.4克/100克体重，p<0.05）。鱼油还可改善HFD诱导的血液、肝脏和脂肪组织脂质代谢失衡，包括血浆和肝脏总胆固醇（TC）显著降低（血浆-HFD组，113±33.6毫克/分升；HFD+FO组，50.0±5.95毫克/分升，p<0.05；肝脏-HFD组，102±13.0毫克/克肝脏；[校正后]HFD+FO组，86.6±7.81毫克/克肝脏，[校正后]p<0.05）、血液、肝脏和脂肪甘油三酯（TG）（血液-HFD组，52.5±20.4毫克/分升；HFD+FO组，29.8±4.30毫克/分升，p<0.05；肝脏-HFD组，56.2±10.0毫克/克肝脏；[校正后]HFD+FO组，30.3±5.28毫克/克肝脏，[校正后]p<0.05；脂肪-HFD组，614±73.2毫克/克肝脏，[校正后]HFD+FO组，409±334毫克/克脂肪组织，[校正后]p<0.05），以及低密度脂蛋白（HFD组，79.8±40.9毫克/分升；HFD+FO组，16.6±5.47毫克/分升，p<0.05）和极低密度脂蛋白（HFD组，49.7±33.3毫克/分升；HFD+FO组，10.4±3.45毫克/分升，p<0.05），同时粪便TC（HFD组，12.2±0.67毫克/克粪便；[校正后]HFD+FO组，16.3±2.04毫克/克粪便，[校正后]<0.05）和TG（HFD组，2.09±0.10毫克/克粪便；[校正后]HFD+FO组，2.38±0.22毫克/克粪便，[校正后]p<0.05）以及脂肪组织脂蛋白脂肪酶活性显著增加（HFD组，16.6±3.64微摩尔对硝基苯酚；HFD+FO组，24.5±4.19微摩尔对硝基苯酚，p<0.05）。此外，鱼油显著激活了HFD喂养大鼠血液或肝脏中肝脏脂质代谢调节因子（AMPKα和PPARα）的蛋白表达，并显著调节了脂质转运相关信号分子（ApoE、MTTP、ApoB、Angptl4、ApoCIII、ACOX1和SREBPF1）。这些结果表明，补充鱼油可改善HFD诱导的大鼠血液、肝脏和脂肪组织脂质稳态失衡。
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