Research Institute for Sustainable Humanosphere (RISH), Kyoto University, Gokasho, 611-0011 Uji, Japan.
Research Institute for Sustainable Humanosphere (RISH), Kyoto University, Gokasho, 611-0011 Uji, Japan.
Carbohydr Polym. 2018 Jun 15;190:95-102. doi: 10.1016/j.carbpol.2018.02.051. Epub 2018 Feb 19.
The production of biofuels and other chemicals from lignocellulosic biomass is limited by the inefficiency of enzymatic hydrolysis. Here a biomimetic composite material consisting of bacterial cellulose and wood-based hemicelluloses was used to study the effects of hemicelluloses on the enzymatic hydrolysis with a commercial cellulase mixture. Bacterial cellulose synthesized in the presence of hemicelluloses, especially xylan, was found to be more susceptible to enzymatic hydrolysis than hemicellulose-free bacterial cellulose. The reason for the easier hydrolysis could be related to the nanoscale structure of the substrate, particularly the packing of cellulose microfibrils into ribbons or bundles. In addition, small-angle X-ray scattering was used to show that the average nanoscale morphology of bacterial cellulose remained unchanged during the enzymatic hydrolysis. The reported easier enzymatic hydrolysis of bacterial cellulose produced in the presence of wood-based xylan offers new insights to overcome biomass recalcitrance through genetic engineering.
木质纤维素生物质生产生物燃料和其他化学品受到酶解效率低下的限制。在这里，使用由细菌纤维素和基于木材的半纤维素组成的仿生复合材料来研究半纤维素对半纤维素酶混合物进行酶解的影响。在半纤维素，特别是木聚糖存在下合成的细菌纤维素比无半纤维素的细菌纤维素更容易受到酶解。更容易水解的原因可能与底物的纳米结构有关，特别是纤维素微纤维成束或成束的排列。此外，小角 X 射线散射表明，在酶解过程中，细菌纤维素的平均纳米形态保持不变。报道称，在木基木聚糖存在下生产的细菌纤维素更容易进行酶解，这为通过基因工程克服生物质抗降解性提供了新的见解。
