Miyanishi T, Horiuti K, Endo M, Matsuda G
Department of Biochemistry, Nagasaki University School of Medicine, Japan.
Eur J Biochem. 1988 Jan 15;171(1-2):31-5. doi: 10.1111/j.1432-1033.1988.tb13754.x.
An antibody was developed against a 23-kDa fragment of myosin which contains a part of the ATP-binding site, and applied to skinned muscle fibers. The antibody abolished active tension generation of the fibers, but did not block their assumption of a rigor state nor their release from this state by ATP. The primary amino acid sequence of the antigenic site on the fragment was found to be the region containing residues 77-80. This sequence region is predicted to have interesting secondary structures, and is distinct from the proposed ATP-binding site. We discuss the possibility that the region is responsible for the energy-transducing step in muscle contraction.
一种针对肌球蛋白23 kDa片段（该片段包含部分ATP结合位点）产生的抗体被应用于去皮肌纤维。该抗体消除了纤维的主动张力产生，但并未阻止其进入僵直状态，也未阻止其通过ATP从该状态释放。发现该片段上抗原位点的一级氨基酸序列是包含77 - 80位残基的区域。该序列区域预计具有有趣的二级结构，且与提议的ATP结合位点不同。我们讨论了该区域负责肌肉收缩中能量转换步骤的可能性。
