肌球蛋白亚片段2抗体对肌纤维收缩力的抑制作用。
Suppression of contractile force in muscle fibers by antibody to myosin subfragment 2.
作者信息
Lovell S, Karr T, Harrington W F
机构信息
Department of Biology, Johns Hopkins University, Baltimore, MD 21218.
出版信息
Proc Natl Acad Sci U S A. 1988 Mar;85(6):1849-53. doi: 10.1073/pnas.85.6.1849.
Abstract
Polyclonal antibody directed against the subfragment-2 region of myosin was purified by affinity chromatography. Skinned muscle fibers that had been preincubated with antibody were able to sustain only 7% of the active isometric force generated by control fibers. The effect of antibody on force production could not be accounted for by inhibition of ATP turnover.
摘要
通过亲和层析法纯化了针对肌球蛋白亚片段-2区域的多克隆抗体。预先用该抗体孵育的去皮肌纤维只能维持对照纤维产生的主动等长力的7%。抗体对力产生的影响不能用ATP周转的抑制来解释。
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