Han Song-Hee, Kim Sung-June, Yun Young Won, Nam Sang Yoon, Lee Hu-Jang, Lee Beom-Jun
College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju, Korea.
College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University, Chinju, Korea.
Lab Anim Res. 2018 Mar;34(1):37-43. doi: 10.5625/lar.2018.34.1.37. Epub 2018 Mar 22.
This study was performed to investigate the effect of a concentrate of fermented wild ginseng root culture (HLJG0701) on memory improvement in the scopolamine (SPL)-induced memory-deficient mouse model. Eight-week-old male ICR mice were used to evaluate the protective effect of HLJG0701 against the SPL-induced memory loss animal model. The Morris water maze test, which measures hippocampus-dependent learning ability, and the Y-maze test, a short-term memory assessment test, were performed and related markers were analyzed. HLJG0701-treated groups displayed significantly reduced acetylcholinesterase activity and increased acetylcholine level compared with the SPL-administered group (SPL-G) (<0.05). In the Y-maze test, the spontaneous alternation in al HLJG0711-treated groups was significantly increased compared with that in SPL-G (<0.05). In the Morris water maze test, the escape latency and time spent in the target quadrant in all HLJG0701-treated groups were significantly decreased and increased, respectively, compared with those in SPL-G (<0.05). In addition, the brain-derived neurotrophic factor level in groups treated with HLJG0701 300 and 600 mg/kg body weight was significantly increased compared with that in SPL-G (<0.05). These results suggest that the HLJG0701 may protect against memory loss by inhibiting acetylcholinesterase activity and preventing acetylcholine deficiency.
本研究旨在探讨发酵野生人参根培养物浓缩物（HLJG0701）对东莨菪碱（SPL）诱导的记忆缺陷小鼠模型记忆改善的影响。使用8周龄雄性ICR小鼠评估HLJG0701对SPL诱导的记忆丧失动物模型的保护作用。进行了测量海马依赖性学习能力的莫里斯水迷宫试验和短期记忆评估试验Y迷宫试验，并分析了相关标志物。与给予SPL的组（SPL-G）相比，HLJG0701治疗组的乙酰胆碱酯酶活性显著降低，乙酰胆碱水平升高（<0.05）。在Y迷宫试验中，与SPL-G组相比，所有HLJG0711治疗组的自发交替显著增加（<0.05）。在莫里斯水迷宫试验中，与SPL-G组相比，所有HLJG0701治疗组的逃避潜伏期显著缩短，在目标象限花费的时间显著增加（<0.05）。此外，与SPL-G组相比，体重300和600mg/kg的HLJG0701治疗组的脑源性神经营养因子水平显著升高（<0.05）。这些结果表明，HLJG0701可能通过抑制乙酰胆碱酯酶活性和预防乙酰胆碱缺乏来预防记忆丧失。
