Chen Hui, Wu Yanqi, Chen Zhu, He Nongyue
J Nanosci Nanotechnol. 2017 Jan;17(1):568-72. doi: 10.1166/jnn.2017.12387.
Integrated automation in laboratory has recruited many traditional standalone devices provided by various vendors, however, because of semi-automatization or lack of standard control interfaces, current available devices are difficult to be integrated into workstation. This paper reported a full-automated thermal cycler system (AutoTCS) equipped with a typical 96-well thermo-cycler module, and especially with a well-designed sliding hot lid for polymerase chain reaction (PCR), which makes it very suitable for using in an automatic nucleic acid testing (NAT) workstation. By a PC software or the touch screen on AutoTCS with user-friendly graphical user interface (GUI), the presented system can not only serve as a PCR module of a NAT workstation, but also can be treated as a single PCR system. Experiments have demonstrated that the AutoTCS achieved a temperature accuracy of ±0.1 °C, a heating rate of approximately 2.5 °C/sec, and cooling rate of approximately 1.6 °C/sec. The AutoTCS was tested with PLCE1 gene and hepatitis B virus (HBV) DNA, and this had successfully achieved an excellent PCR performance.
实验室中的集成自动化纳入了许多由不同供应商提供的传统独立设备，然而，由于这些设备存在半自动化或缺乏标准控制接口的问题，目前可用的设备难以集成到工作站中。本文报道了一种全自动热循环仪系统（AutoTCS），它配备了一个典型的96孔热循环模块，特别是配有一个设计精良的用于聚合酶链反应（PCR）的滑动热盖，这使得它非常适合在自动核酸检测（NAT）工作站中使用。通过PC软件或AutoTCS上具有用户友好图形用户界面（GUI）的触摸屏，该系统不仅可以作为NAT工作站的PCR模块，还可以被视为一个单独的PCR系统。实验表明，AutoTCS的温度精度达到±0.1°C，加热速率约为2.5°C/秒，冷却速率约为1.6°C/秒。使用PLCE1基因和乙型肝炎病毒（HBV）DNA对AutoTCS进行了测试，并且成功实现了出色的PCR性能。
