Department of Marine Life Sciences, Jeju National University, Jeju, 63243, Republic of Korea; Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Department of Pharmaceutical Engineering, Soonchunhyang University, Asan, 31538, Republic of Korea.
Chem Biol Interact. 2018 May 1;287:27-31. doi: 10.1016/j.cbi.2018.04.001. Epub 2018 Apr 6.
Obesity is a serious health issue in many industrialized countries. It is a medical condition with excessive levels of fat accumulated in adipocytes. The objective of the present study was to determine the inhibitory effect of 3-chloro-4,5-dihydroxybenzaldehyde (CDB) on adipogenesis in 3T3-L1 adipocyte cells. CDB suppressed the differentiation and decreased lipid accumulation and triglycerides contents in 3T3-L1 adipocytes. Its suppression effect on fat accumulation was mediated via expression of adipogenesis factors (C/EBPα, SREBP-1c, PPARγ, and adiponectin) during adipocyte differentiation in white adipocyte cells. CDB's ability to suppress fat accumulation was increased in a concentration-dependent manner. It inhibited fatty acid synthesis related proteins including FAS, FABP4, leptin, and perilipin. It also increased expression of phosphorylated AMPK in adipocytes cells. These observations suggest that CDB has potential anti-obesity effect with ability to improve metabolic diseases.
肥胖是许多工业化国家的一个严重健康问题。它是一种医学病症，表现为脂肪细胞中积累了过多的脂肪。本研究的目的是确定 3-氯-4,5-二羟基苯甲醛（CDB）对 3T3-L1 脂肪细胞脂肪生成的抑制作用。CDB 抑制分化，并减少 3T3-L1 脂肪细胞中的脂质积累和三酰甘油含量。其对脂肪积累的抑制作用是通过在白色脂肪细胞的脂肪细胞分化过程中表达脂肪生成因子（C/EBPα、SREBP-1c、PPARγ 和脂联素）来介导的。CDB 抑制脂肪积累的能力呈浓度依赖性增加。它抑制脂肪酸合成相关蛋白，包括 FAS、FABP4、瘦素和 perilipin。它还增加了脂肪细胞中磷酸化 AMPK 的表达。这些观察结果表明，CDB 具有改善代谢疾病的潜在抗肥胖作用。
