Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan.
Virus Res. 2018 May 2;250:31-36. doi: 10.1016/j.virusres.2018.04.005. Epub 2018 Apr 6.
Mosquito-borne alphaviruses are disseminated globally and cause febrile illness in humans and animals. Since the prevalence and diversity of alphaviruses has not been previously investigated in Zambia, reverse transcription PCR was employed as a broad-spectrum approach for the detection of alphaviruses in mosquitoes. From 552 mosquito pools, a novel alphavirus, tentatively named Mwinilunga alphavirus (MWAV), was discovered from a single Culex quinquefasciatus mosquito pool. The full genome of MWAV was subsequently determined, and pairwise comparisons demonstrated that MWAV represented a new alphavirus species. Phylogenetic analyses and a linear discriminant analysis based on the dinucleotide ratios in various virus sequences indicated that MWAV is related to a mosquito-specific alphavirus distinct from other known mosquito-borne alphaviruses due to its inability to replicate in vertebrate cell lines. Further analyses of these novel alphaviruses will help to facilitate a greater understanding of the molecular determinants of host range restriction and the evolutionary relationships of alphaviruses.
虫媒黄病毒在全球范围内传播，并导致人类和动物发热疾病。由于先前在赞比亚尚未调查过黄病毒的流行情况和多样性，因此采用逆转录 PCR 作为一种广谱方法来检测蚊子中的黄病毒。从 552 个蚊子池中，从单个库蚊池中发现了一种新型黄病毒，暂定名为姆温利朗加黄病毒 (MWAV)。随后确定了 MWAV 的全长基因组，并且成对比较表明 MWAV 代表了一种新的黄病毒种。系统发育分析和基于各种病毒序列中二核苷酸比的线性判别分析表明，MWAV 与一种特定于蚊子的黄病毒有关，与其他已知的虫媒黄病毒不同，因为它不能在脊椎动物细胞系中复制。对这些新型黄病毒的进一步分析将有助于更好地了解宿主范围限制的分子决定因素和黄病毒的进化关系。
