Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, Jiangsu, PR China; Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou 215011, PR China.
Jiangsu Key Laboratory of Anaerobic Biotechnology, School of Environmental and Civil Engineering, Jiangnan University, Wuxi 214122, Jiangsu, PR China.
J Environ Manage. 2018 Jul 1;217:478-485. doi: 10.1016/j.jenvman.2018.03.103. Epub 2018 Apr 6.
Volatile fatty acids (VFAs) production from waste activated sludge fermentation could be improved in anaerobic dynamic membrane bioreactor (ADMBR) by retaining residual organics within the reactor and prolonging their reaction time. However, the accumulation of refractory organics made it operate unstably. Therefore, protease catalysis was adopted to deeply exploit those refractory organics in sludge. By combining with dynamic membrane retention, protease catalysis indeed presented a good performance. VFAs yield was further enhanced by over 40% in ADMBR. Membrane fouling was slightly relieved due to protein and polysaccharide degradations in the sludge of dynamic membrane. It was also interestingly found that not only protease activity of sludge was improved from 5 to 21 U/ml, but also β-GLC activity was enhanced from 13 to 20 μmoL/L/h. Microbial community analysis showed protease addition could reduce bacterial richness and evenness in sludge, and accelerate the growth of polysaccharides-hydrolyzing bacteria, as well as inhibit some proteolytic bacteria.
挥发性脂肪酸（VFAs）可通过在厌氧动态膜生物反应器（ADMBR）中保留反应器内的残留有机物并延长其反应时间来提高废活性污泥发酵中的产量。然而，难降解有机物的积累使其运行不稳定。因此，采用蛋白酶催化来深度开发污泥中的这些难降解有机物。通过与动态膜截留相结合，蛋白酶催化确实表现出了良好的效果。VFAs 的产量在 ADMBR 中进一步提高了 40%以上。由于动态膜污泥中蛋白质和多糖的降解，膜污染也得到了轻微缓解。有趣的是，不仅污泥中的蛋白酶活性从 5 提高到了 21 U/ml，β-GLC 活性也从 13 提高到了 20 μmoL/L/h。微生物群落分析表明，添加蛋白酶可以降低污泥中的细菌丰富度和均匀度，并促进多糖水解细菌的生长，同时抑制一些蛋白酶细菌。
