Koyama Renata, Roberto Sergio R, de Souza Reginaldo T, Borges Wellington F S, Anderson Mauri, Waterhouse Andrew L, Cantu Dario, Fidelibus Matthew W, Blanco-Ulate Barbara
Department of Agronomy, Londrina State University, Londrina, Brazil.
Embrapa Grape and Wine, Bento Gonçalves, Brazil.
Front Plant Sci. 2018 Mar 26;9:323. doi: 10.3389/fpls.2018.00323. eCollection 2018.
Hybrid ( ×) table grape cultivars grown in the subtropics often fail to accumulate sufficient anthocyanins to achieve good uniform berry color. Growers of table grapes in temperate regions generally use ethephon and, more recently, ()--abscisic acid (-ABA) to overcome this problem. The objective of this study was to determine if -ABA applications at different timings and concentrations have an effect on anthocyanin regulatory and biosynthetic genes, pigment accumulation, and berry color of the Selection 21 cultivar, a new × hybrid seedless grape that presents lack of red color when grown in subtropical areas. Applications of -ABA 400 mg/L resulted in a higher accumulation of total anthocyanins and of the individual anthocyaninsanthocyanins: delphinidin-3-glucoside, cyanidin-3-glucoside, peonidin-3-glucoside, and malvidin-3-glucoside in the berry skin and improved the color attributes of the berries. Treatment with two applications at 7 days after véraison (DAV) and 21 DAV of -ABA 400 mg/L resulted in a higher accumulation of total anthocyanins in the skin of berries and increased the gene expression of , , , and and of the and transcription factors in the seedless grape cultivar.
种植在亚热带地区的杂交（×）鲜食葡萄品种往往无法积累足够的花青素，难以实现果实色泽均匀良好。温带地区的鲜食葡萄种植者通常使用乙烯利，以及最近使用的（）-脱落酸（-ABA）来解决这个问题。本研究的目的是确定在不同时间和浓度下施用-ABA是否会对‘Selection 21’品种的花青素调控和生物合成基因、色素积累以及果实颜色产生影响，‘Selection 21’是一种新的×杂交无核葡萄品种，在亚热带地区种植时缺乏红色。施用400 mg/L的-ABA导致浆果表皮中总花青素以及单个花青素（花青素：飞燕草素-3-葡萄糖苷、矢车菊素-3-葡萄糖苷、芍药素-3-葡萄糖苷和锦葵素-3-葡萄糖苷）的积累更高，并改善了浆果的颜色属性。在转色期（DAV）后7天和21天施用两次400 mg/L的-ABA处理，导致无核葡萄品种浆果表皮中总花青素的积累更高，并增加了、、、以及和转录因子的基因表达。
