Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA.
Department of Microbiology and Plant Biology, University of Oklahoma, Norman, Oklahoma 73019, USA.
Analyst. 2018 Apr 30;143(9):2008-2011. doi: 10.1039/c7an02065a.
We report a great feature of open tubular liquid chromatography when it is run using an extremely narrow (e.g., 2 μm inner diameter) open tubular column: more than 10 million plates per meter can be achieved in less than 10 min and under an elution pressure of ca. 20 bar. The column is coated with octadecylsilane and both isocratic and gradient separations are performed. We reveal a focusing effect that may be used to interpret the efficiency enhancement. We also demonstrate the feasibility of using this technique for separating complex peptide samples. This high-resolution and fast separation technique is promising and can lead to a powerful tool for trace sample analysis.
我们报告了当使用极细（例如内径 2μm）开管柱进行开管液相色谱时的一个显著特点：在不到 10 分钟的时间内，在约 20 巴的洗脱压力下，可以实现超过 1000 万板/米的柱效。该柱用十八烷基硅烷涂覆，并且进行等度和梯度分离。我们揭示了一种聚焦效应，可用于解释效率的提高。我们还展示了该技术用于分离复杂肽样品的可行性。这种高分辨率和快速分离技术很有前途，可以成为痕量样品分析的有力工具。
