Atopkin D M, Sokolov S G, Shedko M B, Vainutis K S, Orlovskaya O M
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the RAS, pr. 100-letija, 159, Vladivostok, 690022, Russia.
Department of Cell Biology and Genetics, Far Eastern Federal University, 8 Sukhanova Street, Vladivostok, 690051, Russia.
Parasitol Res. 2018 Jun;117(6):1765-1772. doi: 10.1007/s00436-018-5858-y. Epub 2018 Apr 10.
Phylogenetic relationship reconstruction and taxonomical analysis of trematodes of the genus Bunodera was carried out using 28S rDNA partial sequences along with a description and molecular characterisation of a new species, B. vytautasi sp. nov. A new species is reported from the intestine of Pungitius pungitius (Linnaeus, 1758), collected from Magadan Region, Russia. The diagnostic characters of B. vytautasi sp. nov. are the extension of vitelline fields from the oral sucker or posterior edge of the pharynx to the posterior extremity of the body, the confluence of vitelline fields within the forebody, the extension of the cirrus-sac to the posterior third of ventral sucker or further backwards with an outlet beyond the posterior edge of the sucker, and the presence of a unipartite internal seminal vesicle. Both Bayesian and Maximum Likelihood trees shared the same tree topology, in which the genus Bunodera was shown to be monophyletic. Representatives of the genus Bunodera were distributed into three well-supported clades: percid-infecting species (Eurasian species B. luciopercae and B. acerinae and North American B. luciopercae s.l.), gasterosteid-infecting species (amphi-Pacific B. mediovitellata and North American B. inconstans and B. eucaliae) and percid/gasterosteid-infecting species (Asiatic B. vytautasi sp. nov. and North American B. sacculata). Eurasian B. luciopercae and B. acerinae were more closely related to each other than to North American B. luciopercae s.l.
利用28S rDNA部分序列对Bunodera属吸虫进行了系统发育关系重建和分类分析，并对新物种B. vytautasi sp. nov.进行了描述和分子特征分析。报道了从俄罗斯马加丹地区采集的尖头潘氏鱼（Pungitius pungitius，林奈，1758）肠道中发现的一个新物种。B. vytautasi sp. nov.的诊断特征包括：卵黄腺区从口吸盘或咽后缘延伸至身体后端；卵黄腺区在前体部汇合；贮精囊延伸至腹吸盘后三分之一处或更向后，开口超出吸盘后缘；存在单室内部精囊。贝叶斯树和最大似然树具有相同的拓扑结构，其中Bunodera属显示为单系类群。Bunodera属的代表分为三个得到充分支持的分支：感染鲈科鱼类的物种（欧亚物种B. luciopercae和B. acerinae以及北美B. luciopercae s.l.）、感染刺鱼科鱼类的物种（环太平洋地区的B. mediovitellata以及北美的B. inconstans和B. eucaliae）以及感染鲈科/刺鱼科鱼类的物种（亚洲的B. vytautasi sp. nov.和北美的B. sacculata）。欧亚的B. luciopercae和B. acerinae彼此之间的亲缘关系比与北美B. luciopercae s.l.的关系更密切。
