Yang Wenguang, Yu Haibo, Wang Yuechao, Liu Lianqing
State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, Liaoning, China.
Methods Mol Biol. 2018;1771:97-105. doi: 10.1007/978-1-4939-7792-5_8.
A considerable number of studies have focused on fabrication of hydrogel microstructures due to its wide applications in tissue engineering, drug delivery, and extracellular matrix construction. Here, we introduce a hydrogel printing method based on UV-induced projection via a digital micromirror device (DMD). Arbitrary microstructures could be fabricated within few seconds (<3) by modulating UV projection using DMD as digital dynamic masks instead of a physical mask, which also offers a high degree of flexibility and repeatability. Furthermore, the ability of PEGDA film to hinder cell adhesion makes it possible to control area over which cells attach.
