用于生物医学应用的微流控球体培养的建立。

Establishment of Microfluidic Spheroid Cultures for Biomedical Applications.

作者信息

Kwapiszewska Karina

机构信息

Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland.

出版信息

Methods Mol Biol. 2018;1771:213-224. doi: 10.1007/978-1-4939-7792-5_17.

DOI:10.1007/978-1-4939-7792-5_17

PMID:29633216

Abstract

Multicellular spheroid is a three-dimensional (3D) cell culture model that mimics cancer tumor environment. Its widespread use for anticancer therapy evaluation is nowadays limited by accessibility of 3D compatible assays. Here, a microfluidic system for spheroid formation, culture and analysis is presented. The system is compatible with standard microplate readers. The microfluidic chip enables long-term 3D cell culture and in situ monitoring of cellular viability. Moreover, design of the assay enables observation of delayed type of toxicity or application of repeated doses of a drug.

摘要

多细胞球体是一种模拟癌症肿瘤环境的三维（3D）细胞培养模型。目前，其在抗癌治疗评估中的广泛应用受到3D兼容检测方法可及性的限制。在此，我们展示了一种用于球体形成、培养和分析的微流控系统。该系统与标准微孔板读数器兼容。微流控芯片能够实现长期的3D细胞培养以及对细胞活力的原位监测。此外，该检测方法的设计能够观察延迟型毒性或重复给药的应用情况。