• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脐血CD133 +造血干细胞向巨核细胞分化过程中的微小RNA微阵列分析

MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells.

作者信息

Houshmand Mohammad, Nakhlestani Hagh Mozhde, Soleimani Masoud, Hamidieh Amir Ali, Abroun Saeed, Nikougoftar Zarif Mahin

机构信息

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Department of Clinical and Biological Sciences, University of Turin, San Luigi Gonzaga Hospital, Orbassano, Italy.

出版信息

Cell J. 2018 Jul;20(2):195-203. doi: 10.22074/cellj.2018.5021. Epub 2018 Mar 18.

DOI:10.22074/cellj.2018.5021
PMID:29633597
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5893291/
Abstract

OBJECTIVES

In order to clarify the role of microRNAs (miRNA) in megakaryocyte differentiation, we ran a microRNA microarray experiment to measure the expression level of 961 human miRNA in megakaryocytes differentiated from human umbilical cord blood CD133+ cells.

MATERIALS AND METHODS

In this experimental study, human CD133+ hematopoietic stem cells were collected from three human umbilical cord blood (UCB) samples, and then differentiated to the megakaryocytic lineage and characterized by flow cytometry, CFU-assay and ploidy analysis. Subsequently, microarray analysis was undertaken followed by quantitative polymerase chain reaction (qPCR) to validate differentially expressed miRNA identified in the microarray analysis.

RESULTS

A total of 10 and 14 miRNAs were upregulated (e.g. miR-1246 and miR-148-a) and down-regulated (e.g. miR- 551b and miR-10a) respectively during megakaryocyte differentiation, all of which were confirmed by qPCR. Analysis of targets of these miRNA showed that the majority of targets are transcription factors involved in megakaryopoiesis.

CONCLUSIONS

We conclude that miRNA play an important role in megakaryocyte differentiation and may be used as targets to change the rate of differentiation and further our understanding of the biology of megakaryocyte commitment.

摘要

目的

为阐明微小RNA（miRNA）在巨核细胞分化中的作用，我们进行了一项miRNA微阵列实验，以测量从人脐带血CD133+细胞分化而来的巨核细胞中961种人类miRNA的表达水平。

材料与方法

在本实验研究中，从三份人脐带血（UCB）样本中收集人CD133+造血干细胞，然后将其分化为巨核细胞系，并通过流式细胞术、集落形成单位分析和倍性分析进行鉴定。随后，进行微阵列分析，接着进行定量聚合酶链反应（qPCR），以验证在微阵列分析中鉴定出的差异表达miRNA。

结果

在巨核细胞分化过程中，分别有10种和14种miRNA上调（如miR-1246和miR-148-a）和下调（如miR-551b和miR-10a），所有这些均通过qPCR得到证实。对这些miRNA靶标的分析表明，大多数靶标是参与巨核细胞生成的转录因子。

结论

我们得出结论，miRNA在巨核细胞分化中起重要作用，可作为改变分化速率的靶标，并加深我们对巨核细胞定向分化生物学的理解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d969/5893291/21051b7a4e31/Cell-J-20-195-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d969/5893291/ca77837873ef/Cell-J-20-195-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d969/5893291/107fa3c92df4/Cell-J-20-195-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d969/5893291/4753340c1d8d/Cell-J-20-195-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d969/5893291/21051b7a4e31/Cell-J-20-195-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d969/5893291/ca77837873ef/Cell-J-20-195-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d969/5893291/107fa3c92df4/Cell-J-20-195-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d969/5893291/4753340c1d8d/Cell-J-20-195-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d969/5893291/21051b7a4e31/Cell-J-20-195-g04.jpg

相似文献

1
MicroRNA Microarray Profiling during Megakaryocyte Differentiation of Cord Blood CD133+ Hematopoietic Stem Cells.脐血CD133 +造血干细胞向巨核细胞分化过程中的微小RNA微阵列分析
Cell J. 2018 Jul;20(2):195-203. doi: 10.22074/cellj.2018.5021. Epub 2018 Mar 18.
2
Megakaryocytic differentiation of CD133+ hematopoietic stem cells by down-regulation of microRNA-10a.通过下调微小RNA-10a使CD133+造血干细胞向巨核细胞分化
Hematology. 2013 Mar;18(2):93-100. doi: 10.1179/1607845412Y.0000000047. Epub 2012 Nov 19.
3
miR-125b modulates megakaryocyte maturation by targeting the cell-cycle inhibitor p19.微小RNA-125b通过靶向细胞周期抑制剂p19来调节巨核细胞成熟。
Cell Death Dis. 2016 Oct 20;7(10):e2430. doi: 10.1038/cddis.2016.288.
4
MYB controls erythroid versus megakaryocyte lineage fate decision through the miR-486-3p-mediated downregulation of MAF.MYB通过miR-486-3p介导的MAF下调来控制红系与巨核系谱系命运决定。
Cell Death Differ. 2015 Dec;22(12):1906-21. doi: 10.1038/cdd.2015.30. Epub 2015 Apr 10.
5
MicroRNA expression in maturing murine megakaryocytes.小鼠巨核细胞成熟过程中的 microRNA 表达。
Blood. 2010 Dec 2;116(23):e128-38. doi: 10.1182/blood-2010-06-292920. Epub 2010 Aug 18.
6
microRNA-22 promotes megakaryocyte differentiation through repression of its target, .microRNA-22 通过抑制其靶基因 ，促进巨核细胞分化。
Blood Adv. 2019 Jan 8;3(1):33-46. doi: 10.1182/bloodadvances.2018023804.
7
Enhanced expression of hepatocyte-specific microRNAs in valproic acid mediated hepatic trans-differentiation of human umbilical cord derived mesenchymal stem cells.丙戊酸介导人脐带间充质干细胞肝向转分化过程中肝细胞特异性微小RNA的表达增强
Exp Cell Res. 2016 May 1;343(2):237-247. doi: 10.1016/j.yexcr.2016.03.015. Epub 2016 Mar 18.
8
[Ex vivo expansion of megakaryocyte progenitor cells for CD133(+) cells derived from human umbilical cord blood].[人脐带血来源的CD133(+)细胞的巨核细胞祖细胞的体外扩增]
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Jun;16(3):645-9.
9
MicroRNA expression profiles in umbilical cord blood cell lineages.脐带血细胞谱系中的 microRNA 表达谱。
Stem Cells Dev. 2010 Jan;19(1):17-26. doi: 10.1089/scd.2009.0071.
10
Effects of CXCR1 and CXCR2 inhibition on expansion and differentiation of umbilical cord blood CD133(+) cells into megakaryocyte progenitor cells.CXCR1 和 CXCR2 抑制对脐血 CD133(+)细胞向巨核细胞祖细胞扩增和分化的影响。
Cytokine. 2011 Aug;55(2):181-7. doi: 10.1016/j.cyto.2011.04.015. Epub 2011 May 8.

引用本文的文献

1
Shedding Light on Targeting Chronic Myeloid Leukemia Stem Cells.揭示靶向慢性粒细胞白血病干细胞的奥秘
J Clin Med. 2021 Dec 11;10(24):5805. doi: 10.3390/jcm10245805.
2
The miRNA Profile in Non-Hodgkin's Lymphoma Patients with Secondary Myelodysplasia.非霍奇金淋巴瘤继发骨髓增生异常患者的 miRNA 谱。
Cells. 2020 Oct 19;9(10):2318. doi: 10.3390/cells9102318.
3
miR-125a-5p regulates megakaryocyte proplatelet formation via the actin-bundling protein L-plastin.miR-125a-5p 通过肌动蛋白束蛋白 L-塑蛋白调节巨核细胞的前血小板形成。

本文引用的文献

1
Mimicking the Acute Myeloid Leukemia Niche for Molecular Study and Drug Screening.模拟急性髓系白血病微环境用于分子研究和药物筛选
Tissue Eng Part C Methods. 2017 Feb;23(2):72-85. doi: 10.1089/ten.TEC.2016.0404. Epub 2017 Jan 13.
2
Megakaryopoiesis and platelet production: insight into hematopoietic stem cell proliferation and differentiation.巨核细胞生成与血小板生成：对造血干细胞增殖和分化的见解
Stem Cell Investig. 2015 Feb 14;2:3. doi: 10.3978/j.issn.2306-9759.2015.02.01. eCollection 2015.
3
Large-scale production of megakaryocytes from human pluripotent stem cells by chemically defined forward programming.
Blood. 2020 Oct 8;136(15):1760-1772. doi: 10.1182/blood.2020005230.
4
[miR-593 inhibits proliferation of colon cancer cells by down-regulating PLK1].[微小RNA-593通过下调 polo 样激酶1抑制结肠癌细胞增殖]
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Feb 28;39(2):144-149. doi: 10.12122/j.issn.1673-4254.2019.09.03.
5
MiR-9 Controls Chemotactic Activity of Cord Blood CD34⁺ Cells by Repressing CXCR4 Expression.微小RNA-9通过抑制CXCR4表达来控制脐血CD34⁺细胞的趋化活性。
Int J Stem Cells. 2018 Nov 30;11(2):187-195. doi: 10.15283/ijsc18057.
通过化学限定的正向编程从人多能干细胞大规模生产巨核细胞。
Nat Commun. 2016 Apr 7;7:11208. doi: 10.1038/ncomms11208.
4
Overexpression and knockout of miR-126 both promote leukemogenesis.miR-126的过表达和敲除均促进白血病发生。
Blood. 2015 Oct 22;126(17):2005-15. doi: 10.1182/blood-2015-04-639062. Epub 2015 Sep 11.
5
MicroRNA Processing and Human Cancer.微小RNA加工与人类癌症
J Clin Med. 2015 Aug 21;4(8):1651-67. doi: 10.3390/jcm4081651.
6
Upregulation of miR-181s reverses mesenchymal transition by targeting KPNA4 in glioblastoma.在胶质母细胞瘤中，miR-181s的上调通过靶向核转运蛋白α4（KPNA4）逆转间充质转化。
Sci Rep. 2015 Aug 18;5:13072. doi: 10.1038/srep13072.
7
Serum miRNA expression profile as a prognostic biomarker of stage II/III colorectal adenocarcinoma.血清微小RNA表达谱作为II/III期结直肠癌的预后生物标志物
Sci Rep. 2015 Aug 7;5:12921. doi: 10.1038/srep12921.
8
Tissue engineering and microRNAs: future perspectives in regenerative medicine.组织工程与微小RNA：再生医学的未来展望
Expert Opin Biol Ther. 2015;15(11):1601-22. doi: 10.1517/14712598.2015.1071349. Epub 2015 Jul 22.
9
The roles of microRNAs in the regulation of tumor metastasis.微小RNA在肿瘤转移调控中的作用。
Cell Biosci. 2015 Jun 20;5:32. doi: 10.1186/s13578-015-0028-8. eCollection 2015.
10
MYB controls erythroid versus megakaryocyte lineage fate decision through the miR-486-3p-mediated downregulation of MAF.MYB通过miR-486-3p介导的MAF下调来控制红系与巨核系谱系命运决定。
Cell Death Differ. 2015 Dec;22(12):1906-21. doi: 10.1038/cdd.2015.30. Epub 2015 Apr 10.